Project background Papers of interest:

Chibby: Shi, J., Y, Zhao, D. Galati, M. Winey & M.W. Klymkowsky. 2014. Chibby functions in Xenopus ciliary assembly, embryonic development, and the regulation of gene expression. Developmental Biology, 395: 287–298.

Chibby drives β catenin cytoplasmic accumulation leading to activationof the unfolded protein response in BCR-ABL1+ cells

BAR Domain-Containing FAM92 Proteins Interact with Chibby1 To Facilitate Ciliogenesis

Centrin: Shi, J., Y. Zhou, T. Vonderfecht, M. Winey & M.W. Klymkowsky. 2015. Centrin-2 mediated regulation of FGF/FGFR gene expression in Xenopus. Scientific Reports, 5:10283. doi: 10.1038/srep10283.

EFHC1: Zhao, Y., J. Shi, M. Winey & M.W. Klymkowsky. 2016. Identifying domains of EFHC1 involved in axonemal localization, ciliogenesis, and the regulation of Wnt signaling. Dev. Biol. 411: 257-65.

VIMENTIN: Mice lacking vimentin develop and reproduce without an obvious phenotype

A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus.

DESMIN: Disruption of intermediate filament organization leads to structural defects at the intersomite junction in Xenopus myotomal muscle.

METHODS:
BioID: Identifying protein-protein associations at the nuclear envelope with BioID.
Split-BioID: a conditional proteomics apporach to monitor the compositions of spatiotemporally defined protein complexes.

TURBO-ID: Directed evolution of turboID for efficient proximity labeling in living cells and organisms.
& Ting lab AddGene site

The Cellular Thermal Shift Assay: a novel biophysical assay for in situ drug targe engegement and mechanistic biomarker studies.