Clouds, now & then,
giving relief from constant
moon-viewing - Bashò

Table of contents
 
Immuno-
cytochemistry
Oocyte
isolation
Embryo
injection
SDS PAGE &
Immunoblots
Whole-mount
(Alcian and such)

2-D  gels
1D IEF analysis

embryo nucleus
isolation - Xenopus

In situ probe
synthesis

Embryo lysates &
Immunoprepicipitation

Plasmid minipreps | Empirical miniprep
Wax- & cryo
sectioning
Making competant
bacteria
In situ
hybridization

MiniPreps for
making RNA

Plasmid construction: PCR and subcloning

in vitro
protein synthesis
Xenopus ChIP Posters in
powerpoint
Making RNA from
Xenopus

Green plasmids

Whole-mount
TUNEL

Neural crest
transplants

DNA fishing
in Xenopus
PhotoFishing
and IP
zeb
XENBASE Miscellaneous
recipes

Expansion
Microscopy (web)

Brefeldin & Polio


low cost-weekend free protocol (soon)

iPSC cells workshop
day 1 & day 2
maxi-quiagen
protocol)
iPSC CRISPR
mutation  protocol (soon)
crispr-mutation plasmid (soon)

last update - 11 March 2022