Plasmid Name: pAdeno.Rdmyc                                                      Stock #: 1

 

Strain:DH5α

Description: full coding region of rat desmin cDNA subcloned into the modified pAd.CMV vector. Used for making adenovirus

Created by: Klymkowsky-Oct 95
Checked: March 2010

Plasmid Name: pAdeno.mycEp-CADTAIL.Myc                           Stock #: 2

 

 Strain:DH5α

Description: myc Xenopus EP-Cadherin Tail domain.myc inserted into pAdeno for making adenvirus. pAdCMV. Defective

Created by: Klymkowsky-Oct 95-Box 2
Checked: March 2010

Plasmid Name: pAdeno.mycDGlTAIL.Myc                                 Stock #: 3

 

Strain:DH5α

Description: the bovine desmoglein tail domain with tags at each end, inserted into the pAdeno vector

Created by: Klymkowsky-Oct 95-Box 1
Checked: March 2010

 

 

Plasmid Name: pT7*RDM-1                                                         Stock #: 4

 

Strain:DH5α

Description: contains a mutated form of the rat desmin cDNA in which there is a predicated dominant mutation from Arg to Cys at the beginning of the rod region. This construct was made using the PCR overlap; it will be sequenced to confirm the mutation.

Created by: Klymkowsky-Nov 95-Box 1
Checked: March 2010

Plasmid Name: pAdeno.Dsc1b.myc                                             Stock #: 5

 

Strain:DH5α

Description: contains a mutated form of the rat desmin cDNA in which there is a predicated dominant mutation from Arg to Cys at the beginning of the rod region. This construct was made using the PCR overlap; it will be sequenced to confirm the mutation.

Created by: Klymkowsky-Nov 95-Box 1
Checked: March 2010

 

 

 

Plasmid Name: pAd.CMV.cat.D                                                 Stock #: 6

 

Strain:DH5α

Description: original adenovirus plasmid for construction of replication defective virus

Created by: Kass-Eisler and Leinward
Checked: March 2010

Plasmid Name: pAdeno.RDM-3                                                 Stock #: 7

 

Strain:DH5α

Description: Dominant mutation of rat desmin (myc) Met 262 changed to Arg 262

Created by: Damon Thomas
Checked: March 2010

Plasmid Name: pAdeno. Dsc1b.myc                                          Stock #: 8

Strain:DH5α

 Description: a recessive mutation in the rat desmin sequence, apparent generated during PCR amplification. Note: according to Bob Evans it does not assemble correctly in IF-free cells but does co-assemble with vimentin Created by: The great unknowable PCR spirit
Checked: March 2010

Plasmid Name: pAdeno.XDesmin.myc                                      Stock #: 9

Strain:DH5α

 Description: the full length xenopus desmin cDNA with myc tag. Derived from plasmids used in the Cary & Klymowsky papers. I think that this plasmid has only a single Bam Hl site; it is derived from a second plasmid with a second Bam HI site in the promoter. We are currently testing whether the removal of this site effects expression.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*mycXPg                                                   Stock #: 10

Strain:DH5α

 Description: encodes an N-Terminally tagged, full length version of Xenopus Plakoglobin.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*m Head+CR PKG                                    Stock #: 11

Strain:DH5α

 Description: PCR derived mutation of Xenopus Plakoglobin in which tail domain is removed.

Created by: Dana Dodd-1994

Checked: March 2010

Plasmid Name:pT7*mCR PKG                                                Stock #: 12

Strain:DH5α

 Description: PCR derived mutation of Xenopus Plakoglobin in which head and domains have been removed.

Created by: Dana Dodd- Klymkowsky lab

Checked: March 2010

Plasmid Name:pT7*mCR+Tail PKG                                        Stock #: 13

Strain:DH5α

 Description: PCR derived mutation of Xenopus Plakoglobin in which head domain is removed.

Created by: Dana Dodd- Klymkowsky lab

Checked: March 2010

Plasmid Name:pSK.GFP                                                                   Stock #: 14

 Strain:DH5α

 Description: plasmid containing coding sequence for Fluorescent green protein. –original protein (not mutated)

Created by: ?

Checked: March 2010

Plasmid Name:pT7*mycDgITail                                      Stock #: 15

 Strain:DH5α            

 Description: human desmoglobin-1 tail domain with a N-terminal myc tag

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*mycDgITail (untagged)                    Stock #: 16

Strain:DH5α           

 Description: an untagged form of the desmoglobin tail domain

Created by: Alla Karnovsky-Klymkowsky Lab

Checked: March 2010

Plasmid Name:pT7*mycDsc1b.Tail.myc                         Stock #: 17

 Strain:DH5α           

 Description: the 1b tail domain of bovine desmocollin 1b with a C-terminal myc-tag

Created by: Jose Nieves

Checked: March 2010

Plasmid Name:pT7*mycDsc1a.Tail.myc                         Stock #: 18

Strain:DH5α                        

 Description: the 1a tail domain of bovine desmocollin 1 with a C-terminal myc-tag

Created by: Alla Karnovsky-Klymkowsky Lab

Checked: March 2010

Plasmid Name:pT7*mycCadtail                                       Stock #: 19 Strain:DH5α                        

 Description: the cytoplasmic tail domain of Xenopus EP-© cadherin with an N-terminal myc-tag

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pAd*CMV.cat.D                                      Stock #: 20 Strain:DH5α                         

 Description: a version of the original pAd.CMV.cat.D plasmid with an upstream (unique) Nde I site removed. Tested by Alison Kass-Eisler and found to be active transcriptionally. 

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pSP6.mycXmango                                    Stock #: 21

Strain:DH5α                    

 Description: Plasmid contains a myc-taggged form of the Xenopus mago nashi cDNA for synthesis and injection of RNA.

Created by: Phil Newmark-Boswell lab

Checked: March 2010

Plasmid Name:pT7*Dsc3a.Tail.myc                                Stock #: 22

Strain:DH5α                        

 Description: the (human) desmocollin -3a tail domain with a C-terminal myc tag

Created by: Alla Karnovsky-Klymkowsky Lab

Checked: March 2010

Plasmid Name: pT7*mDglTail.GFP (old)                        Stock #: 23

Strain:DH5α                          

 Description: a chimera of the GFP polypeptide linked to the C-terminus of the mycDgTail region. In this plasmid the 3’UTR has been deleted!

Created by: Jurgen Schmeinsky-Klymkowsky lab

Checked: March 2010

Plasmid Name:pT7*mDglTail.GFP (new)                       Stock #: 24

Strain:DH5α                          

 Description: GFP region contains mutation Ser65-Thr65 ; said to make the fluorescence brighter, faster and better in every way. Note: This plasmid also does not have a 3’UTR.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pSP6.Hum.GSK3B                                   Stock #: 25

Strain:DH5α                          

 Description: wild type of GSK3b in an SP6 transcription plasmid. Supplied by HE et all (Varmus & Dawid)

Created by: HE - (Varmus & Dawid)

Checked: March 2010

Plasmid Name:pSP6.Hum.GSK3B (dominant)                Stock #: 26

Strain:DH5α                                       

 Description: dominant mutant form of GSK3b in an SP6 transcription plasmid. Supplied by HE et all (Varmus & Dawid)

Created by: HE - (Varmus & Dawid)

Checked: March 2010

Plasmid Name:pHPGCa2.1                                              Stock #: 27

Strain:DH5α                                      

 Description: full length cDNA clone of Human Plakoglobin (from Werner Franke) in the pBluescript KS+plasmid. Insert length 3.21 kbp. Accession number M23410—confirmed by EcoRI digest

Created by: Michaela Goldschimidt-Franke lab

Checked: March 2010

Plasmid Name:pHPGCa2.1                                              Stock #: 28

Strain:DH5α                                        

 Description: a full length (somewhat rearranged at the 5’ end) cDNA of Rat desmin.

Created by: Guido Swart

Checked: March 2010

Plasmid Name:pCS2-mt-Mouse Axin                              Stock #: 29

Strain:DH5α                                          

 Description: this is the orginal RDM-1 mutation that I planned. It contains a two amino acid change of Asp116.Arg117 to Glu116.Cys117. Mutated version contains a new Bsm1 site in the region of the mutation.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pACTII                                                     Stock #: 30

Strain:DH5α                                          

 Description: the plasmid (from Steve Elledge) that normally contains the cDNA library cloned downstream of the RNA ploymerse activator domain of Gal4

Created by: Elledge et al

Checked: March 2010

Plasmid Name:pACTII-X/N                                             Stock #: 31

Strain:DH5α                                         

Description: A modified version of pACTII in which an Xho I and Not I linker has been inserted in order to accept the Xenopus stage 30 cDNA library. Confirmed by sequencing.

Created by: Mark Driver- Klymkowsky lab

Checked: March 2010

Plasmid Name:pAS.CYH                                                 Stock #: 32

Strain:DH5α                               

Description: The shuttle vector used in the yeast two-hybrid system. Contains DNA binding domain of GAL4 with downstream prey.

Created by: Elledge et al

Checked: March 2010

Plasmid Name:pAS.DesminHead.myc                             Stock #: 33 Strain:DH5α                                        

Description: the head of xenopus desmin linked to the DNA-binding domain of GAL4 in the yeast shuttle vector pAS.CYH

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pAS.Vimhead.myc                                   Stock #: 34

Strain:DH5α                                        

Description: the head of vitemntin in yeast two-hybrid plasmid pAS.CYH

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pAS.Dsc1b.myc                                        Stock #: 35

Strain:DH5α                                       

Description: the tail domain of bovine desmocollin 1b.myc in the yeast two-hydrid vector

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pCS2-mt-Mouse Axin                              Stock #: 36                            

Description: the full length mouse Axin subcloned into pCS2mt

Created by: Li Zeng-Constantini lab

Checked: March 2010

Plasmid Name:pCS2-mt-Mouse Axin Δ-RGS                 Stock #: 37

Strain:DH5α                                        

Description: this is a domiany mutant of mouse Axin in the pCS2mt plasmid missing the RGS domain

Created by: Li Zeng-Constantini lab

Checked: March 2010

Plasmid Name:pAS.mXMago                                          Stock #: 38

Strain:DH5α                                        

Description: this is a myc-tagged form of the Xenopus mago nashi cDNA(isolated by the Boswell lab) placed in the pAS.CYH Plasmid. It does not activate LacZ gene expression on its own.

Created by: Adam Rubenstien- Klymkowsky lab

Checked: March 2010

Plasmid Name:pT7*mycXPkg.4+10                                Stock #: 39

Strain:DH5α                              

Description: this plasmid contains arm repeats 4 to 10 of Xen. Plakoglobin subcloned behind the myc tag 

Created by: Adam Rubenstien- Klymkowsky lab

Checked: March 2010

Plasmid Name:pAdeno.XenDesmin.myc                         Stock #: 40

Strain:DH5α                                       

Description: a step on the way to rest of the pAdeno plasmids, has 2 BamHI sites

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name: No Plasmid                                              Stock #: 41

Strain:GM2163                                       

Description: this is a dam/dcm-strain from NEB for manipulating plasmids with methylase sensitive restriction sites

Created by: NEB

Checked: March 2010

Plasmid Name: No Plasmid                                              Stock #: 42

Strain:XL-1 Blue                                       

Description: Bacterial cell strain

Created by: mystery

Checked: March 2010

Plasmid Name: No Plasmid                                              Stock #: 43

Strain:DH5α                                                                                                                                                                                                                                                                            

Description: Standard cells for transformation

Created by: -

Checked: March 2010

Plasmid Name: No Plasmid                                              Stock #: 44

Strain:XL2-Blue                                       

Description: stratagene’s supercompetant cells

Created by: stratagene

Checked: March 2010

Plasmid Name: No Plasmid                                              Stock #: 45

Strain:JM109                                       

Description: for transforming with bacteria

Created by: -

Checked: March 2010

Plasmid Name:pXenopus Plakoglobin                             Stock #: 46

Strain:JM103                                                      

Description: original xen. PKG cDNA from Berdadette Foquet and Werner Franke

Created by: Berdadette Foquet and Werner Franke

Checked: March 2010

Plasmid Name:pXenopus EP ©-Cadherin                        Stock #: 47

Strain:JM103                                                      

Description: a full length cDNA cloned of Xenopus C-Cadherin Werner Franke

Created by: Barry Gumbiner et al

Checked: March 2010

Plasmid Name:pXT-Xbra                                                 Stock #: 48

Strain:JM103                                                      

Description: the xenopus brachyury cDNA in a plasmid that can be used to make anti-sense probe for in situ hybridization

Created by: Unknown

Checked: March 2010

Plasmid Name:pT7*Xvimentin-1.myc                              Stock #: 49

Strain:JM103                           

Description: the full coding sequence of xenopus vimentin-1 with a C-terminal tag.

Created by: Leilah Backhus- Klymkowsky lab

Checked: March 2010

Plasmid Name:pSP6.XVimentin-2.myc                            Stock #: 50

Strain:JM109

Description: the full coding sequence of Xenopus vimentin-2 with a C-terminal myc tag in the pSP64T plasmid.

Created by: Joseph Dent-Klymkowsky lab

Checked: March 2010

Plasmid Name:pET.XVimentin-1.stop                              Stock #: 51

Strain:BL21 DE3 pLysS                                                                                                                                                                                                                                                                                                      

Description: Bacterial protein synthesis vector containing vimentin-1 coding sequence with a stop codon (to tag)

Created by: Alberto Domingo-Klymkowsky lab

Checked: March 2010

Plasmid Name:pH7-“goosecoid” in Jm 103                   Stock #: 52A

Strain:DH5α                                                                                                                                                                                                                                                                           

Description: this is the wild type Xenopus GSK3b from David Kimelman

Created by: David Kimelman

Checked: March 2010

Plasmid Name:pSP6.XGsk3(wild type)                         Stock #: 52B

Strain:DH5α                                                                                                                                                                                                                                                                            

Description: this is the wild type Xenopus GSK3b from David Kimelman

Created by: David Kimelman

Checked: March 2010

Plasmid Name:pSP6.XGsk.Dominant Mutation                Stock #: 53

Strain:DH5α                                                                                                                                                                                                                                                                            

Description: a dominant mutation of Xenopus Gsk3

Created by: David Kimelman

Checked: March 2010

Plasmid Name:pT7*Bov.Desmocollin-1b.full.myc         Stock #: 54

 Strain:DH5α                                                                                                                                                                                                                                                                           

Description: this is a full length cDNA of desmocollin 1b (myc-tag)

Created by: Alla Karnovsky-Klymkowsky lab

Checked: March 2010

Plasmid Name:pET.mycSDesminTail                               Stock #: 55

Strain:DH5α                                                                                                                                                                                                                                                                            

Description: this is a plasmid, proportedly sequenced by RB Cary and correct in the protein Xba I and EcoRI sites are in the coding region.

Created by: RB Cary-Klymkowsky lab

Checked: March 2010

Plasmid Name:pET.mycVimTail                                       Stock #: 56

Strain:DH5α                                                                                                                                                                                                                                                                            

Description: Xenopus vimentin-1’s tail domain.

Created by: RB Cary-Klymkowsky lab

Checked: March 2010

Plasmid Name:pT7*mycXPkg.FGP                                  Stock #: 57

 Strain:DH5α                                                                                                                                                                                                                                                                           

Description: mutated form of the FGP protein in frame at C-terminus of XPKG

Created by: Pam Yenkinson-Klymkowsky lab

Checked: March 2010

Plasmid Name:pSP6.XKl.tag in Jm103                          Stock #: 58A

Strain:XL2                                                                                                                                                                                                                                               

Description: this is the RMD-3 mutation in which M 262 is changed to an Arg; made by the two-PCR/overlap method. Needs to be confirmed by sequencing.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*RDM-3                                           Stock #: 58B

Strain:XL2                                                                                                                                                                                                                                             

Description: this is the RMD-3 mutation in which M 262 is changed to an Arg; made by the two-PCR/overlap method. Needs to be confirmed by sequencing.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*mycDesminTail                                 Stock #: 59

Strain:DH5α                                                                                                                                                                                                                                                 

Description: this is the new DesminTail construct subcloned into pT7*. It will be checked by TnT reaction and subcloned into pAS.CYH.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pAdeno RDM-3                                     Stock #: 60A

Strain:DH5α                                                                                                                                                                                                                                                 

Description: construct made in the Leinwand Lab, based on VR1012 plasmid with CMV 1E promoter /enchancer and CMV 1E’UTR together with “BGH terminal” region. Contains the FGP mutation that I made inserted with Xba I and BamHI sites.

Created by: Rob-Leinwand Lab

Checked: March 2010

Plasmid Name:pAdeno RDM-3                                     Stock #: 60B

Strain:DH5α                                                                                                                                                                                                                      

Description: construct made in the Leinwand Lab, based on VR1012 plasmid with CMV 1E promoter /enchancer and CMV 1E’UTR together with “BGH terminal” region. Contains the FGP mutation that I made inserted with Xba I and BamHI sites.

Created by: Rob-Leinwand Lab                                   

Checked:  March 2010

Plasmid Name:pT7*Xdesmin Head.myc                           Stock #: 61

Strain:DH5α                                                                                                                                                                                                                      

Description: This is the desmin head region recovered from yeast cells by plasmid rescue. To be used in screening of two hybrid library.

Created by: Mark Driver-Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pAS.CYH.mycXDesmin tail                     Stock #: 62

Strain:DH5α                                                                                                                                                                                                                       

Description: this is the newly made Desmin Tail domain inserted into the pAS.CYH plasmid for two hybrid screening. Insert is 240b

Created by: Mark Driver-Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pSP6.B catinen (HA)                                 Stock #: 63

Strain:DH5α                                                                                                                                                                                                                       

Description: this is the Xenopus b-catenin RNA synthesis plasmid from Barry Gumbiner the protein is HA tagged. To linearize, cut with EcoR1

Created by: Gimbiner lab

Checked: March 2010

Plasmid Name:pcDNA3                                                     Stock #: 64

Strain:DH5α                                                                                                                                                                                                                      

Description: adenovirus promoter/enhancer primer

Created by: invitrogen

Checked: March 2010

Plasmid Name:pT7*C-cadherin anchor –part 1                 Stock #: 65

Strain:DH5α                                                                                                                                                                                                                    

Description: this is the first part in the construction of a membrane-anchored form of plakoglobin-contains signal and N-terminal domain of Xenopus C-cadherin.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*myc ΔN.XPkg                                   Stock #: 66

Strain:DH5α                                                                                                                                                                                                                    

Description: a truncation of XPKG up to the beginning of the putative GSK site (homology domain 1)

Created by: M.W. Klymkowsky-95

Checked: March 2010

Plasmid Name:pT7*myc Cadtail.FGP                               Stock #: 67

Strain:DH5α                                                                                                                                                                                                                    

Description: this is the Xenopus EP-cadherin tail plasmid, linked in frame to the mutated form of FGP; is also has normal UTRs

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pConnexin                                                  Stock #: 68

Strain:DH5α                                                                                                                                                                                                                    

Description: this is a pSK derived (T3 and T7) that encodes the transmembrane domains of rat connexin-32 linked in frame to synaptopysin.

Created by: Rudolf Leube-Franke Lab

Checked: March 2010

Plasmid Name:pT7*mycCG.Xpkg                                    Stock #: 69 Strain:DH5α                                                                                                                                                                                                                  

Description: deletion of the GSK concensus site from the head of XpKG

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pET3a*mycDG81Tail                               Stock #: 70

Strain:BL21(DE3) pLysS                                                                                                                                                                                                                     

Description: this is the tail domain of the type 1 Keratin DG81A with a N-terminal myc-tag placed in the bacterial expression vector PET3a

Created by: Robert Cary-Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pET3a*XVimentin-1.GFP(old)                 Stock #: 71

Strain:BL21(DE3)                                                                                                                                                                                                                     

Description: this encodes a chimera of xenopus vimentin and FGP. Has been checked and acts as a dominant mutation in vitro

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name: pET.Xdesmin.Stop                                    Stock #: 72

Strain:BL21(DE3)                                                                                                                                                                                                          

Description: this plasmid expresses the full length Xenopus desmin polypeptide in bacteria

Created by: Robert Cary-Klymkowsky lab                       

Checked: March 2010

Plasmid Name: pET.Xenopus XK1 myc                            Stock #: 73

Strain:BL21(DE3)                                                                                                                                                                                                                     

Description: this plasmid expresses the type 2 xenopus Keratin 8 homolog (with a myc-tah) in bacteria.

Created by: Alberto Domingo/Jeff Bachant-Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pET3a.XVimentin-1.Myc                        Stock #: 74

Strain:BL21(DE3)                                                                                                                                                                                             

Description: plasmid expresses a myc-tagged form of Xenopus vimentin-1

Created by: Joe Dent and Robert Cary -Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pET.XDesmin.myc                                    Stock #: 75

Strain:DH5α                                                                                                                                                                                            

Description: this plasmid encodes a myc-tagged form of Xenopus desmin

Created by: Robert Cary -Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pET.XDesminn.myc.FGP                          Stock #: 76

Strain:DH5α                                                                                                                                                                                         

Description: Plasmid expresses xenopus desmin-FGP Chimera 

Created by: Pam Yenkinson -Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pAdeno.mycCadtail.FGp                           Stock #: 77

Strain:DH5α                                                                                                                                                                                         

Description: the mutated FGP attached inframe to the myoCadtail sequence in the adenovirus vector. 

Created by: Pam Yenkinson -Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pAdeno.HPkg.myc                                    Stock #: 78

Strain:DH5α                                                                                                                                                                                         

Description: Full length human plakoglobin sequence, with a C-Terminal myc tag, in the pAdeno plasmid for eukaryotic expression/adenovirus construction 

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*HPkg.myc                                         Stock #: 79

Strain:DH5α                                                                                                                                                                                         

Description: Full length human plakoglobin sequence, with a C-Terminal myc tag, functions to induce dorsal mesoderm in the early Xenopus embryo

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*Cnx.HPkg.myc                                  Stock #: 80

Strain:DH5α                                                                                                                                                                                         

Description: this is the rat connexin transmembrane domain inserted at the N-terminus of Human Plakoglobin-contains an EcoRI site to facilitate subcloning of other N-Terminal myc-tagged cDNAs as required.

Created by: M.W. Klymkowsky-1996

Checked: March 2010

Plasmid Name:pET myc-cys-Vim Tail                              Stock #: 81

 Strain:DH5α                                                                                                                                                                                        

Description: this is the vimentin tail with a cys-inserted between the myc-tag and tail to dimerize it. Made with an undergrade whose name now escapes me

Created by: M.W. Klymkowsky

Checked: March 2010

 

Plasmid Name:pAdeno.Rat.Desmin-myc                          Stock #: 82

Strain:DH5α                                                                                                                                                                                         

Description: wile type rat desmin for virus production.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pB4inteqrin-Total                                      Stock #: 83

Strain:DH5α                                                                                                                                                                                         

Description: this plasmid contains the entire coding sequence of human b4 integrin

Created by: Vito Quarata Lab

Checked: March 2010

Plasmid Name:pT7*Cnx.HPkg.FGP                                 Stock #: 84

Strain:DH5α                                                                                                                                                                                        

Description: this plasmid encodes a connexin transmembrane domain, human plakoglobin fluorescent green protein (mutated version) chimeric polypeptide.

Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pT7*HPkg.FGP                                         Stock #: 85

Strain:DH5α                                                                                                                                                                                         

Description: this plasmid encodes a human plakoglobin-FGP chimera Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pET.XK1.FGP                                           Stock #: 86

Strain:BL21 (DE3) pLysS                                                                                                                                                                                         

Description: this is the keratin-FGP construct made by Jurgen schmeinsky during his time in the lab, there is no 3’ BamHI site—dead end ligation Created by: Jurgen schmeinsky

Checked: March 2010

Plasmid Name:pAdeno.Rat.Desmin(w.t).FGP                  Stock #: 87

 Strain:DH5α                                                                                                                                                                     

Description: in frame chimera of Rat Desmin.FGP

 Created by: M.W. Klymkowsky

Checked: March 2010

Note:missing

Plasmid Name:pCS2-XSox3                                              Stock #: 88

Strain:DH5α                                                                                                                                                                      

Description: from A Zorn/ no tag

 Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pAdeno.RDM-2.myc                                 Stock #: 89

Strain:DH5α                                                                                                                                                              

Description: this is the RDM-2 mutation, subcloned into the pAdeno vector. It needs to be sequenced by Kurt to be confirmed

 Created by: Kurt Harbold and M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pET.RatDesmin.Wildtype.myc                  Stock #: 90

Strain:DH5α                                                                                                                                                  

Description: rat desmin.myc subcloned into the pET plasmid for bacterial expression

 Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pET.RDM-3                                               Stock #: 91

Strain:DH5α                                                                                                                                                 

Description: RDM-3.myc subcloned into the pET plasmid for bacterial expression

 Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pET.RDM-4                                               Stock #: 92

 Strain:DH5α                                                                                                                                                

Description: RDM-4.myc subcloned into the pET plasmid for bacterial expression

 Created by: M.W. Klymkowsky

Checked: March 2010

Plasmid Name:pSynaptoHPkg                                           Stock #: 93

Strain:DH5α                                                                                                                                                  

Description: this plasmid encodes the transmembrane domain of synaptophysin, linked to the human plakoglobin

 Created by: S.Troyanovsky

Checked: March 2010

Plasmid Name:pT7Dsc1b-FGP                                          Stock #: 94

Strain:DH5α                                                                                                                                                  

Description: this is a Dsc1b-FGP chimera

 Created by: Adam Ruberstein

Checked: March 2010

Plasmid Name:pRecXenopus Fibronectin                         Stock #: 95

 Strain:DH5α                                                                                                                                                 

Description: plasmid contains xenopus fibronectin ina bacterial expression plasmid

 Created by: Doug Desimone

Checked: March 2010

Plasmid Name:pT7HPq8Δ-myc                                         Stock #: 96

 Strain:DH5α                                                                                                                               

Description: truncation of the HPkg coding sequence after the 8th arm repeat

Created by: Yanni Yourgouraskis-Klymkowsky lab                       

Checked: March 2010

Plasmid Name:pZyxin                                                        Stock #: 97

Strain:DH5α                                                                                                                                

Description: to fill

 Created by: mary beckerle                       

Checked: March 2010

Plasmid Name:pT7*CAnchor (no myc)                             Stock #: 98

 Strain:DH5α                                                                                                             

Description: to fill

 Created by: Adam Ruberstein/Mike Klymkowsky                        

Checked: March 2010

Plasmid Name:pT7*Syn (no myc)                                     Stock #: 99

 Strain:DH5α                                                                                           

Description: to fill

 Created by: Adam Ruberstein/Mike Klymkowsky                       

Checked: March 2010

Plasmid Name:pT7*CadXTM (no myc)                         Stock #: 100

 Strain:DH5α                                                                                           

Description: this is the entire signal, extracellular and transmembrane domains of Xenopus C-cadherin in the pT7* plasmid with no myc sequence, there is a 3’Nde 1 site for subcloning.

 Created by: Mike Klymkowsky                       

Checked: March 2010

Plasmid Name:pT7*CadXTM (no myc)                         Stock #: 101

Strain:JM103                                                                                            

Description: this is the entire signal, extracellular and transmembrane domains of Xenopus C-cadherin in the pT7* plasmid with no myc sequence, there is a 3’Nde 1 site for subcloning.

 Created by: Mike Klymkowsky                       

Checked: March 2010

Plasmid Name:pT7*SynHPkg-myc                                 Stock #: 102

Strain:JM103                                                                              

Description: this is the N-terminal,4 transmembrane portion of rat synaptpphysin (ala troyanovsk) attached to the HPKG-myc sequence

 Created by: Mike Klymkowsky                       

Checked: March 2010

Plasmid Name:pT7*CadXTM-HPkg-myc                      Stock #: 103

Strain:DH5α                                                                                            

Description: this is the N-terminal region of C-cadherin (minus the cytoplasmic tail) attached to the HPKg-myc sequence. 

 Created by: Mike Klymkowsky                       

Checked: March 2010

Plasmid Name:pT3XnF7-1                                          Stock #: 104A

Strain:DH5α                                                                         

Description: contains NLS sequence of Xnf7-1

 Created by: Adam Rudenstein                       

Checked: March 2010

Plasmid Name:pT7*HPkg-NLS.myc                            Stock #: 104b

Strain:DH5α                                                                          

Description: contains NLS sequence of Xnf7-1

 Created by: Adam Rudenstein                       

Checked: March 2010

Plasmid Name:pT7*HPkg-NES-myc                              Stock #: 105

 Strain:DH5α                                                                         

Description: contains nuclear exclusion sequence of zyxin

 Created by: Adam Rudenstein/ Mike Klymkowsky                       

Checked: March 2010

Plasmid Name:pT7*mycVimTail-myc                            Stock #: 106

Strain:DH5α                                                                       

Description: this is the double tagged tail of xenopus vimentin tail (Cary et al 1994)

 Created by: Robbert Carry Klymkowsky                        

Checked: March 2010

Plasmid Name:pCsCnx ΔGXBCatenin                           Stock #: 106

Strain:DH5α                                                                       

Description: this is the double tagged tail of xenopus vimentin tail (Cary et al 1994)

 Created by: Robbert Carry-Klymkowsky                       

Checked: March 2010

Plasmid Name:pCsCnx ΔGXBCatenin                           Stock #: 107

Strain:DH5α                                                                      

Description: None

 Created by: Klymkowsky                       

Checked: March 2010

Plasmid Name: None listed                                              Stock #: 108

 Strain:Unknown                                                                     

Description: None

 Created by:                    

Checked: March 2010

Plasmid Name: pGoosecoid                                             Stock #: 109

 Strain:DH5α                                                                     

Description: This plasmid is used to make probes for in situ analysis

 Created by: DeRobertis lab                    

Checked: March 2010

Plasmid Name: pT7*Sal-VimentinTail-myc                   Stock #: 110

 Strain:DH5α                                                                    

Description: under construction

-used to facilitate the subcloning of plasmids into the pCskAct

 Created by: Mark Driver Klymkowsky lab                     

Checked: March 2010

Plasmid Name: pT7*Cite-VimentinTail-myc                  Stock #: 111

Strain:DH5α                                                       

Description: under construction

This is a cap-independent translation enchacer plasmid

 Created by: Mike Klymkowsky               

Checked: March 2010

Plasmid Name: pT7*Canchor-myc                                  Stock #: 112

Strain:DH5α                                         

Description: This is a plasmid that was constructed by piecing together parts of C-cadherin.. It has a 3’ myc-tag.

 Created by: Mike Klymkowsky               

Checked: March 2010

Plasmid Name: pXNF7-1                                                 Stock #: 113

 Strain:DH5α                                        

Description: this is a plasmid that contains pXNF7 cDNA with number of mutations such that there is an NLS sequence at the 5’end. Used to construct the pT7HPkg-NLS-myc plasmid

 Created by: Larry Etkin et al

Checked: March 2010

Plasmid Name: pT7Canchor-HPkg-myc (second)          Stock #: 114

Strain:DH5α                                         

Description: this is the original canchor (part 1+2) region, subcloned from pT7canchor-myc into the pT7HPkg-myc plasmid (no second PCR)

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7(sal-minus)mycVTmyc                    Stock #: 115

 Strain:DH5α                                        

Description: this plasmid has a site near the BAMHI site, removed to facilitate subcloning into pCskACT plasmid

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pCS2-MT                                                Stock #: 116

Strain:DH5α                                         

Description: this plasmid has a Sal I NDE I-GFP-myc-BamHI insert (no Xba I site) in the pT7* Sal plasmid—can be used in making GFP RNA of for subcloning into on the way to pCskACT

Created by: David turner

Checked: March 2010

Plasmid Name: pXotch                                                    Stock #: 117

Strain:DH5α                           

Description: plasmids contain full encoding sequence of Xenopus Notch

 Created by: Clark Coffman

Checked: March 2010

Plasmid Name: pCS2-XotchpDBD-UGP                        Stock #: 118

Strain:DH5α                 

Description: contains c-terminal (putative) disheveled-binding domain of Xenopus Notch within frame UGP in the CS2 plasmid

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7*mycΔG13ΔXPkg                           Stock #: 119

Strain:DH5α                 

Description: this is a deletion after the 13th arm repeat of the DG form of Xenopus plakoglobin

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7*mycΔG10ΔXPkg                           Stock #: 120

 Strain:DH5α                

Description: deletion of GSK site, deletion following the 10th arm repeat.

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7*mycΔG6ΔXPkg                             Stock #: 122

Strain:DH5α             

Description: deletion of GSK site, deletion following the 6th arm repeat.

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7*mycΔG5ΔXPkg                             Stock #: 123

Strain:DH5α                 

Description: deletion of GSK site, deletion following the 5th arm repeat.

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7*Sal-NotB4                                       Stock #: 124

 Strain:DH5α                

Description: contains C-terminal domain of human b4 integrin tail domain

 Created by: Mike Mazzini Mike Klymkowsky

Checked: March 2010

Plasmid Name: pT7cite-mycΔGC8Δ.XPkg-myc            Stock #: 125

Strain:DH5α             

Description: this plasmid contains the cap-independent translation enhancer upstream of the mycΔGC8Δ.XPkg-myc coding sequence; needs to be tested for translational efficiency—is corrected by TnT reaction.      

 Created by: Mike Klymkowsky

Checked: March 2010

Plasmid Name: pCS2mtXotch-DBD (stop)--Check        Stock #: 126

 Strain:DH5α          

Description: this plasmid contains the C-terminus of Xenopus notch in a pT7* plasmid (with 5’tag-myc).      

 Created by: Yanni Vourgourakis-Klymkowsky lab

Checked: March 2010

Plasmid Name: pT7*mycDsmHead                                Stock #: 127

 Strain:DH5α          

Description: a N-Terminally tagged version for the yeast 2-hybrid system—to be subcloned into pAS.CYH

 Created by: Mark Driver-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*mycDsmHead-myc                        Stock #: 128

Strain:DH5α        

Description: a second version of the DsmHead-myc plasmid for subcloning into pAS.CYH

 Created by: Mark Driver-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-Arrowhead-UGP                      Stock #: 129

 Strain:DH5α       

Description: Full coding sequence of arrowhead (for Heilig) in the pCS2 plasmid with 5’ myc and 3’ UGP tags

 Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mt-Arrowhead-UGP                      Stock #: 130

 Strain:DH5α       

Description: this is a yeast “bait” plasmid with the myc-tag on the N-terminus of the desmin head polypeptide, between desmin’s head and the Gal4DNA binding domain.

 Created by: Mark Driver-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*HPkg-C5 Δ-myc                             Stock #: 131

 Strain:DH5α       

Description: part of the human PKG deletion series

 Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pAS1-HPkg-C5Δ-myc.CYH                  Stock #: 132

Strain:DH5α        

Description: the C5Δ deletion of human plakoglobin in the yeast bait-plasmid.

 Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*-HPkg-C5Δ-myc                            Stock #: 133

Strain:DH5α     

Description: part of the C-terminal human PKG truncation series

 Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*-HPkg-C10Δ- myc                         Stock #: 134

Strain:DH5α    

Description: part of the C-terminal truncation human PKG

 Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*mvcHpkg-N2A-SuperGP               Stock #: 135

Strain:DH5α    

Description: part of the N-terminal deletion series of human PKG causes severe adhesion defect when expressed in the early embryo (appears around gastrulation) 

 Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*mvcHpkg-N2 Δ-SGP                     Stock #: 136

 Strain:DH5α 

Description: part of the N-terminal deletion series of human PKG

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*mvcHpkg-N5Δ-SGP                      Stock #: 137

 Strain:DH5α 

Description: part of the N-terminal deletion series of human PKG

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*mvcHpkg-N6Δ-SGP                      Stock #: 138

Strain:DH5α   

Description: part of the N-terminal deletion series of human PKG causes severe adhesion defect when expressed in the early embryo (appears around gastrulation) 

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pSK.SuperGreenProtein                         Stock #: 139

Strain:DH5α   

Description: plasmid recovered from Clark Coffman contains S-T mutation in GFP

Created by: Mike Klymkowsky and Clark Coffman

Checked: April 2010

Plasmid Name: pSK.UltraGreen Protein                         Stock #: 140

Strain:DH5α 

Description: contains S-A mutation; Bgl II site removed Serves as PCR substrate for subcloning into other plasmids (under construction)

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pT7TS-HA-XSox17B                             Stock #: 141

Strain:DH5α 

Description: plasmids encoding HA tagged, full length XSox17 beta sequence. Linearize with Pst1 to make RNA. Injection of RNA ventralizes early Xenopus embryo.

Created by: Aaron Zorn-Gurdon LAB

Checked: April 2010

Plasmid Name: pCS2*mt                                                 Stock #: 142

Strain:DH5α 

Description: BamHI site in the original pCS2-MT plasmid has been removed

Created by: Yanni Vourgourakis-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-SGP                                          Stock #: 143

Strain:DH5α 

Description: super green protein in the pCS2*-MT plasmid 

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2*mt-UGP                                       Stock #: 144

Strain:DH5α 

Description: Ultra green protein in the un-modified pCS2 plasmid

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2*mt-ΔG8ΔXpkg                             Stock #: 145

Strain:DH5α 

Description: DNA/RNA expression plasmid for analysis 

Created by: Yanni Vourgourakis-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*myc-UGP                                       Stock #: 146

Strain:DH5α 

Description: expression plasmid with 5’ myc and 3*S-A mutant in GFP 

Created by: Rachel DeMent-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*mycHPkg-N7Δ-GFP                      Stock #: 147

Strain:DH5α 

Description: part of the deletion series for HPKG 

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-XPkg-UGP                               Stock #: 148

Strain:DH5α 

Description: full length Xenopus plakoglobin in the pCS2 plasmid  

Created by: Chris Dutton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pT7*myc NOTB4 Tail                           Stock #: 149

Strain:DH5α 

Description: the not b4 tail region of b4 integrin (human) for subcloning into pACT II- to check desmin/integrin interactions

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mt-HARM5-8-UGP                       Stock #: 150

Strain:DH5α 

Description: human PKG arm repeats 4-8 in the pCS2-UGP vector

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mt-HARM5-8-6-UGP                   Stock #: 151

Strain:DH5α 

Description: human PKG arm repeats 4-8 in the pCS2-UGP vector

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pHuman Plakophilin-A1                        Stock #: 152

Strain:DH5α 

Description: Full length cDNA of human plakophilin 1A from Franke Lab

Created by: Franke et al

Checked: April 2010

Plasmid Name: pBand6 protein                                       Stock #: 153

Strain:DH5α 

Description: this is a partial cDNA (not complete) isolated by Wheelock and Johnson by PCR

Created by: Johnson & Wheelock

Checked: April 2010

Plasmid Name: pT7*mycHPkg-N5Δ-HPg                      Stock #: 154

Strain:DH5α 

Description: deleted from the N-terminus through the 5th ARM repeat

Created by: Adam Ruberstein

Checked: April 2010

Plasmid Name: pT7*mycHPkg-N7Δ-HPg                      Stock #: 155

Strain:DH5α 

Description: deleted from the N-terminus through the 7th ARM repeat

Created by: Adam Ruberstein

Checked: April 2010

Plasmid Name: pT7*mycHARM 4-10-SGP                    Stock #: 156

Strain:DH5α 

Description: contains arm repeats 4 through 10 of human plakoglobin with an attached SGP

Created by: Adam Ruberstein

Checked: April 2010

Plasmid Name: pCS2-mt-HPlakophilin-UGP                 Stock #: 157

Strain:DH5α 

Description: this is a full length plakoglobin with a 5’ 6X myc tag and a 3’UGP sequence

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pT7*mycHPlpn                                      Stock #: 158

Strain:DH5α 

Description: this is the full length human plakophilin cDNA in the T7 plasmid

Created by: Mike Klymkowsky-confirmed by Adam Ruberstein

Checked: April 2010

Plasmid Name: pCS2myc-NH3-SGP                              Stock #: 159

Strain:DH5α 

Description: in this plasmid, myc GFP moiety is upstream of the desired coding sequence. Unfortunatly, I forgot to include a stop codon after the Xba I site

Created by: Mike Klymkowsky- Adam Ruberstein

Checked: April 2010

Plasmid Name: pCS2myc-NH3-SGP                              Stock #: 160

Strain:DH5α 

Description: the complete tail of Xotch with a 5’6myc sequence and a 3’ UGP moiety.

Created by: Yanni Vourgourakis Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mycUGP-XotchDBD                     Stock #: 161

Strain:DH5α 

Description: the putative “disheleveled-binding domain” of Xotch with a 5’ myc-GFP tag “ultra form”

Created by: Yanni Vourgourakis–Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mycUGP-Xotch Total tail             Stock #: 162

Strain:DH5α 

Description: this is the complete Xotch ctyoplastic tail domain with a 5’myc GFP (ultra) tag.

Created by: Yanni Vourgourakis–Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-HPlpn-GFP                               Stock #: 163

Strain:DH5α 

Description: plakophilin ready

Created by: Jayme Sanders–Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-hAPC-25-GFP                          Stock #: 164

Strain:DH5α 

Description: APC25 with a 3’ GFP

Created by: Chris Dutton–Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mycGFP-HAPC25                        Stock #: 165

Strain:DH5α 

Description: this is the APC25 sequence with a 5’myc-SGP attached

Created by: Chris Dutton- Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2MT-APC25-SGP                           Stock #: 166

Strain:DH5α 

Description: APC25 with a 3’ GFP Tag

Created by: Chris Dutton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pASI.myc.HMPKGN5 Δ.CYH2            Stock #: 167

Strain:DH5α 

Description: removel of the head of HPlpn—attached to the N-terminal mycGFP

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: April 2010

Plasmid Name: pASI.myc.HMPKGN10 Δ.CYH2          Stock #: 168

Strain:DH5α 

Description: keratin promoter (epidermal) from Tom Sargent 

Created by: Tom Sargent (NIH)

Checked: April 2010

Plasmid Name: pASI.myc.HMPKGN7 Δ.CYH2            Stock #: 169

Strain:DH5α 

Description: N-terminal 6x myc-head deleted, Human Pipn-UGP tail 

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: April 2010

Plasmid Name: pAS.N Δ2.PKG                                      Stock #: 170

Strain:DH5α 

Description: yeast 

Created by: adam

Checked: April 2010

Plasmid Name: pCAR.MT-APC25-SGP                         Stock #: 171

Strain:DH5α 

Description: cardiac promoter with APC (3’GFP) construct in

Created by: Chris Dutton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCAR.mycG-APC-25                            Stock #: 172

Strain:DH5α 

Description: cardiac promoter with APC 5’ GFP tagged form of APC25

Created by: Chris Dutton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCAR.mycG-APC-25                            Stock #: 173

Strain:DH5α 

Description: Xenopus cardiac promoter plasmid (original)

Created by: Enrique Amaya (source)

Checked: April 2010

Plasmid Name: pCAR.mycSGP                                      Stock #: 174

Strain:DH5α 

Description: cardiac promoter plasmid with 5’ GFP tag

Created by: Chris Dutton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCAR-MT-SGP                                     Stock #: 175

Strain:DH5α 

Description: cardiac promoter plasmid with 3’ GFP tag—can be used as reporter since GFP includes stop sequence

Created by: Chris Dufton-Klymkowsky lab

Checked: April 2010

Plasmid Name: no information                                        Stock #: 176

Strain:DH5α 

 

Plasmid Name: pCS2mt-XTcf3 (stopped)                       Stock #: 177

Strain:DH5α 

Description: this is a stopped version of the pCS2mt-XTCF-3 plasmid

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pT7.XWnt8-green                                  Stock #: 178

Strain:DH5α 

Description: this should be the xenopus wnt8 attached in the frame to SGP but the Xba I/ BamHI digests do not look right (no digest)

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mt-Head of HPpn-1-UGP              Stock #: 179

Strain:DH5α 

Description: head only of PPN-1 localized to nuclei in A6 

Created by: Jayme Sanders/ Yanni Vourgourakis- Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mycUGP-ΔC5HPkg                      Stock #: 180

Strain:DH5α 

Description: The C5delta construct in pCS2 

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: April 2010

Plasmid Name: pCSmt-HPPn-1-UGP                             Stock #: 181

Strain:DH5α 

Description: Full sequence

Created by: Klymkowsky

Checked: April 2010

Plasmid Name: pGEX4T2                                               Stock #: 182

Strain:DH5α 

Description: this is a gst-fusion plasmid

Created by: commercial-obtained by the amazing JG!

Checked: April 2010

Plasmid Name: pGEX4T3                                               Stock #: 183

Strain:DH5α 

Description: this is another gst-fusion protein plasmid

Created by: commercial-obtained by the amazing JG!

Checked: April 2010

Plasmid Name: pCS2MT-HDesmogleinTail-GFP          Stock #: 184

Strain:DH5α 

Description: this is the desmoglein tail domain placed in the pCS2 plasmid

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pT7*mycRatDesmin head                      Stock #: 185

Strain:DH5α 

Description: the head of rat desmin, used to make pAS.mycRDsmHead

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCsCnxHPkg-GFP                                 Stock #: 186

Strain:DH5α 

Description: this is Cnx anchored plakoglobin (human) in the pCS7 plasmid, with a 3’GFP group-NO MYC SEQUENCE. Plasmid contains to opposing T7 promoters and no SP6

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-DesminHead-UGP                   Stock #: 187

Strain:DH5α 

Description: the desmin head construction in the pCS2 plasmid. I do not know if this is Xenopus or rat or whether there is a 5’ MT. I would check it before I used it.

Created by: Kira Wendorf- Klymkowsky lab

Checked: April 2010

Plasmid Name: pUC8-C1-rat plectin                               Stock #: 188

Strain:DH5α 

Description: this is a protein of rat plectin cDNA

Created by: Gerhard Wiche

Checked: April 2010

Plasmid Name: pAS1.mycRatDesminHead-CYH2         Stock #: 189

Strain:DH5α 

Description: for two hybrid screening 

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: p3.8-N- β-tubulin-CAT                           Stock #: 190

Strain:DH5α 

Description: the Xenopus neural b-tubulin promoter driving CAT 

Created by: Pual Krieg (supplied by Angie Ribera)

Checked: April 2010

Plasmid Name: pT7*mycPROX- β4Tail                         Stock #: 191

Strain:DH5α 

Description: this is the last segment of the human b4 cytoplasmic tail domain. To be subcloned into the pT7*mycScaβ4Tail 

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mG-Dsc1aTail                               Stock #: 192

Strain:DH5α 

Description: a CS2 plasmid with a 5’ mGFP sequence and ready to be tested 

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mG-Dsc1bTail                               Stock #: 193

Strain:DH5α 

Description: a CS2 mycGFP version of Dsc1bTail

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pUC81-Δ13 (epidermal promoter)        Stock #: 194

Strain:DH5α 

Description: this should be the xenopus epidermal promoter region from Tom Sargent

Created by: Tom Sargent/ Chris Dutfon

Checked: April 2010

Plasmid Name: pT7-HA-XTcf-3                                     Stock #: 195

Strain:DH5α 

Description: Molenaar et all-Xenopus TCF-1—like transcription factor.

Created by: Molenaar et al

Checked: April 2010

Plasmid Name: pT7-HA-ΔN-XT-cf-3                             Stock #: 196

Strain:DH5α 

Description: Molenaar et all-Xenopus TCF-1—like transcription factor.

Created by: Molenaar et al

Checked: April 2010

Plasmid Name: pCS2mtXSox-17β-GFP                          Stock #: 197

Strain:DH5α 

Description: an N-Terminal myc-tagged, C-terminal GFP tagged form of XSox 17β

Created by: Klymowsky  

Checked: April 2010

Plasmid Name: pCS2mtXSox-17β                                  Stock #: 198

Strain:DH5α 

Description: an N-Terminal myc-GFP tagged form of XSox 17β

Created by: Klymowsky 

Checked: April 2010

Plasmid Name: pCsCnx-N5Δ-HPkg-GFP                       Stock #: 199

Strain:DH5α 

Description: deletion of head and first four armsof human plakoglobin in the anchored form. Checked in A6 cells and it works very nicely. This is the pSC7 plasmid

Created by: Klymowsky 

Checked: April 2010

Plasmid Name: pCsCnx-N5Δ-HPkg-GFP                       Stock #: 200

Strain:DH5α 

Description: deletion of head and first four armsof human plakoglobin in the anchored form. Checked in A6 cells and it works very nicely. This is the pSC7 plasmid

Created by: Klymowsky 

Checked: April 2010

Plasmid Name: pCS2mt- N5Δ-HPkg-GFP                      Stock #: 201

Strain:DH5α 

Description: contains the n5delta version of HPg in the pCS2mt plasmid

Created by: Adam Ruberstein-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-mycGFP-Proxβ4Tail                Stock #: 202

Strain:DH5α 

Description: this plasmid contains the region of β4 integrin proximal to the plasma membrane and running up to the unique Sca I site

Created by: Mike Mazzini -Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-mycGFP-Notβ4Tail                 Stock #: 203

Strain:DH5α 

Description: this is the C-Terminal end of the β4 integrin rail domain attached to a myc-GFP disrupts IF organization in Xenopus cells by binding to plectin apparently

Created by: Mike Mazzini -Klymkowsky lab

Checked: April 2010

Plasmid Name: pXEX-UGP                                            Stock #: 204

Strain:DH5α 

Description: this is the mt-UGP Version of thee Xenopus elongation factor promoter plasmid. Original XEX plasmid from Paul Krieg

Created by: Chris Dufton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pSK-epidermal promoter                        Stock #: 205

Strain:DH5α 

Description: this is the Xenopus epidermal keratin promoter, subcloned into the pSK plasmid for sequencing. Original plasmid from Tom Sargent

Created by: Chris Dufton-Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mycG-DsgTail                              Stock #: 206

Strain:DH5α 

Description: an N-terminally tagged form of our original mycDsgTail plasmid

Created by: Mike  Klymkowsky

Checked: April 2010

Plasmid Name: pAdeno.N5Δ.HPg-myc                          Stock #: 207

Strain:DH5α 

Description: version of plakoglobin for adenovirus production makes appropriate sized polypeptide

Created by: Lori Alger Klymkowsky lab

Checked: April 2010

Plasmid Name: pLOX-mGFP-Sca-β4Tail                       Stock #: 208

Strain:DH5α 

Description: a plasmid to be tested to make adenoviruses—not much success with this.

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pLOX-mycGFP                                      Stock #: 209

Strain:DH5α 

Description: as in thee case of stock 208

Created by: Mike Klymkowsky

Checked: April 2010

Plasmid Name: pGEX2                                                   Stock #: 210

Strain:DH5α 

Description: this is the modified form of the pGEX plasmid for subcloning into with EcoRI/Xba I  

Created by: Julie Gelderloos, Jarmel Chen- Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-HPpn Head-GFP                      Stock #: 211

Strain:DH5α 

Description: should be the ppn head domain 

Created by:Klymkowsky

Checked: April 2010

Plasmid Name: No information                                       Stock #: 212

Strain: Unknown  

Description:

Created by:

Checked: April 2010

Plasmid Name: pCS2mycProxB1.GFP                           Stock #: 213

Strain: Unknown

Description:

Created by:

Checked: April 2010

Plasmid Name: pT7mScaB4FGP                                    Stock #: 214

Strain: Unknown

Description:

Created by:

Checked: April 2010

Plasmid Name: pEPI-VGP                                              Stock #: 215

Strain:DH5α  

Description: this is the epidermal keratin promoter driving the expression of GFP

Created by: Chris Dufton-Klymkowsky

Checked: April 2010

Plasmid Name: pXEX-CnxHPg-GFP                              Stock #: 216

Strain:DH5α  

Description: this is the epidermal keratin promoter driving the expression of GFP

Created by: Chris Dufton-Klymkowsky

Checked: April 2010

Plasmid Name: pXEX-Cnx N5Δ HPg-GFP                    Stock #: 217

Strain:DH5α

Description: this is the Xenopus elongation factor promoter driving cxn-n5deltaHPg-GFP

Created by: Chris Dufton-Klymkowsky

Checked: April 2010

Plasmid Name: pT7*C-CAD-myc                                   Stock #: 218

Strain: Unknown

Description:

Created by:

Checked: April 2010

Plasmid Name: pSP36T-SP6 C-CAD                              Stock #: 219

Strain: Unknown

Description:

Created by:

Checked: April 2010

Plasmid Name: pCS2mt-XTcf-3-UGP                            Stock #: 220

Strain:DH5α

Description: a 5’ myc tagged form of the XTcf-3 transcription factor with a 3’GFP

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: April 2010

Plasmid Name: pSK-HMG Box                                      Stock #: 222

Strain:DH5α

Description:  the HMG box XTCF3 (I believe) by itself for making probe.  

Created by: Amy Hopkins Klymkowsky lab

Checked: April 2010

Plasmid Name: pCS2mt-N2D-HPg-GFP                        Stock #: 223

Strain:DH5α

Description:  the N2D version of plakoglobin with a 5’ mt and a 3’ GFP. 

Created by: Klymkowsky lab

Checked: April 2010

Plasmid Name: pET-RDM-4                                           Stock #: 224

Strain:?

Description:  the RDM-4 mutation in pET

Created by: Klymkowsky lab

Checked: April 2010

Plasmid Name: pET-RDmyc                                           Stock #: 225

Strain:?

Description: 

Created by: ?

Checked: April 2010

Plasmid Name: pT7- β4 TotalTail-GFP                          Stock #: 226

Strain:Jm109

Description:  total tail domain of b4 integrin 

Created by: Mike Mazzi Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mt-BFP                                          Stock #: 227

Strain:DH5α

Description:  blue fluorescent form of the pCS2mt-plasmid

Created by: Klymkowsky

Checked: April 2010

Plasmid Name: pCS2mt                                                   Stock #: 228

Strain:DH5α

Description:  base plasmid

Created by: Turner & Rupp

Checked: April 2010

Plasmid Name: pCS2mt-X βCatenin                               Stock #: 229

Strain:DH5α

Description:  full length Xenopus bCatenin in the pCS2 plasmid

Created by: Gumbiner lab

Checked: May 2010

Plasmid Name: pCS2mG-Scaβ4                                     Stock #: 230

Strain:DH5α

Description:  N-terminally mycGFP tagged Sca C-terminal fragment of the b4 integrin cytoplasmic tali domain

Created by: Mike Mazzi Klymkowsky

Checked: May 2010

Plasmid Name: pCAR-mG-Scaβ4                                   Stock #: 231

Strain:DH5α

Description:  Scab4 in the cardiac actin promoter plasmid

Created by: Mike Mazzi Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mG-β4 total tail                             Stock #: 232

Strain:DH5α

Description:  the total cytoplasmic tail of b4 integrin in the pCS2mG

Created by: Mike Mazzi Klymkowsky

Checked: May 2010

Plasmid Name: pCS2ΔG-XβCatenin-BFP                      Stock #: 233

Strain:DH5α

Description:  the deltaG form of XbCatenin attached to BFP

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mt-BFP                                          Stock #: 234

Strain:DH5α

Description:  BFP in pCS2mt-subcloned using EcoRI and Xba I

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mt-ΔG-XPg-BFP                           Stock #: 235

Strain:DH5α

Description:  the blue form of delta G-XPg

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mt- DG-XPg-BFP                         Stock #: 236

Strain:DH5α

Description:  the same as stock 235

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pXEX-GFP (5’)                                      Stock #: 237

Strain:DH5α

Description:  the xenopus elongation factor promoter

Created by: Chris Dufton (I think)

Checked: May 2010

Plasmid Name: pFull length human LEF-1                     Stock #: 238

Strain:DH5α

Description:  the orginal human LEF-1 plasmid

Created by: from Kathy Jones

Checked: May 2010

Plasmid Name: pCS2mG-hLEF-1                                   Stock #: 239

Strain:DH5α

Description:  the N-terminal GFP tagged form of hLEF1 could be incorrect-check

Created by:

Checked: May 2010

Plasmid Name: pCS2mt-hLEF-1-G                                 Stock #: 240

Strain:DH5α

Description:  the C-terminal GFP tagged form it is under that this is correct needs to be checked

Created by:

Checked: May 2010

Plasmid Name: pCS2mtRatDesminHead-GFP               Stock #: 241

Strain:DH5α

Description:  the head of rat desmin with a C-terminal GFP

Created by: Kira Wendorf-klymkowsky

Checked: May 2010

Plasmid Name: pRSETB-Dsc1aTail                               Stock #: 242

Strain: Unknown

Description:  Dsc1a tail was modified to fit into pRSETB

Created by: Julie Gelderloos and Jarmei chen-Klymkowsky lab

Checked: May 2010

Plasmid Name: pRSETB                                                 Stock #: 243

Strain: Unknown

Description:  original plasmid

Created by:

Checked: May 2010

Plasmid Name: pRSETC                                                 Stock #: 244

Strain: Unknown

Description:  original plasmid

Created by:

Checked: May 2010

Plasmid Name: pCS2mt-ΔG-XPg                                   Stock #: 245

Strain:DH5α

Description:  the delta G form, no C-Terminal tag

Created by: Chris Dufton 

Checked: May 2010

Plasmid Name: pCS2mtDG-XPg-RT competitor           Stock #: 246

Strain:DH5α

Description:  as small deletion to be used as a competitor in rtPCR quantitation

Created by: Chris Dufton-Klymkowsky lab 

Checked: May 2010

Plasmid Name: pCS2mtΔGXbCATENIN                       Stock #: 247

Strain:DH5α

Description:  as small deletion to be used as a competitor in rtPCR quantitation

Created by: Chris Dufton-Klymkowsky lab 

Checked: May 2010

Plasmid Name:                                                                 Stock #: 248

Strain: Unknown

Description: 

Created by:

Checked: May 2010

Plasmid Name: pRSETb.Xpg in BL21                            Stock #: 249

Strain: Unknown

Description: 

Created by:

Checked: May 2010

Plasmid Name: pSETbDsca1aTail                                  Stock #: 250

Strain:BL21

Description: desmocollin 1a tail domain  

Created by: Amy Hopkins

Checked: May 2010

Plasmid Name: BL21plysS                                              Stock #: 251

Strain:BL21

Description: for protein purification

Created by: Mike Overduin

Checked: May 2010

Plasmid Name: BB34 pLysS                                           Stock #: 252

Strain:BB34

Description: for protein purification

Created by: Mike Overduin

Checked: May 2010

Plasmid Name: pT7*mycLEF-1                                      Stock #: 253

Strain:DH5α

Description: could be incorrect-check before using 

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: May 2010

Plasmid Name: pRSETB-1                                              Stock #: 254

Strain:DH5α

Description: missing Xba I site in original pRSETB plasmid 

Created by: Jamie Chen- Klymkowsky lab

Checked: May 2010

Plasmid Name: pCsCnxHPg-BFP                                   Stock #: 255

Strain:DH5α

Description: anchored-blue plakoglobin   

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pRSETb.XpgpLysS in BB34                 Stock #: 256

Strain: Unknown

Description:

Created by:

Checked: May 2010

Plasmid Name: pRSETb.Dsc1aTail+pLysS                    Stock #: 257

Strain:B834

Description: for protein purification

Created by: Amy Hopkins- Klymkowsky lab

Checked: May 2010

Plasmid Name: pRSETb-Dsc1aTail+pLysS                    Stock #: 258

Strain:BL21 Cells

Description: for protein purification

Created by: Amy Hopkins- Klymkowsky lab

Checked: May 2010

Plasmid Name: pRSETb-2                                               Stock #: 259

Strain:DH5α

Description: this has the original Xba I site removed and an insert added with “in frame” EcoRI and Xba I sites for subcloning

Created by: Jamie Chen- Klymkowsky lab

Checked: May 2010

Plasmid Name: pCS2mt-mNeuroD                                Stock #: 260

Strain:DH5α

Description: is the mouse NeuroD protein in pCS2mt

Created by: Jackie Lee

Checked: May 2010

Plasmid Name: pCS2mtX12a                                          Stock #: 261

Strain:DH5α

Description: an HLH protein

Created by: Jackie Lee

Checked: May 2010

Plasmid Name: pRSETB*-XPg                                       Stock #: 262

Strain:DH5α

Description: the full sequence of xenopus plakoglobin in the bacterial expression plasmid pRSETB* (modified by us)

Created by: Jamei Chen

Checked: May 2010

Plasmid Name: pCS2mtβCAT-competitor                      Stock #: 263

Strain:DH5α

Description: don’t know whats going on here

Created by: Chris Dufton-Klymkowsky lab 

Checked: May 2010

Plasmid Name: pCS2mt-ND166-XTcf3-GFP                 Stock #: 264

Strain:DH5α

Description: this is the N-terminally deleted form of XTcf3-does not bind to catenin and screws up embryos when you inject the RNA (according to Yanni)

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCsCnxΔG-XPg SGP                             Stock #: 265

Strain:DH5α

Description: one of the two is wrong, be careful

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCsCnxN2Δ-HPg-stop                           Stock #: 266

Strain:DH5α

Description: this is the stopped form-needs to be checked!- size of insert is not right

Created by: Klymkowsky lab

Checked: May 2010

Plasmid Name: pCsCnxN5Δ-HPg-stop                           Stock #: 267

Strain:DH5α

Description: this is the stopped form-seems to be ok

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mtΔG-XPg-stop                             Stock #: 268

Strain:DH5α

Description: we have a few of these variants take care to use the right one

Created by: Chris Dufton

Checked: May 2010

Plasmid Name: pCS2mtΔG-XPg-CΔ10-stop                  Stock #: 269

Strain:DH5α

Description: the C-terminally deleted form of the protein

Created by: Chris Dufton

Checked: May 2010

Plasmid Name: pCS2mG-XC-CadTail                            Stock #: 270

Strain:DH5α

Description: the Green form of the C-cadherin tail domain

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mG-XSlug                                     Stock #: 271

Strain:DH5α

Description: a N-terminal xenopus slug

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pRSETb*-XPg +pLysS                          Stock #: 272

Strain:DH5α

Description: for protein purification

Created by: Amy Hopkins

Checked: May 2010

Plasmid Name: pCS2mtΔG-XPg-CΔ13-stop                  Stock #: 273

Strain:DH5α

Description: a C-terminal truncation plasmid

Created by: Chris Dufton

Checked: May 2010

Plasmid Name: pT7*mycXSlug                                      Stock #: 274

Strain:DH5α

Description: a simple myc tagged form of slug

Created by: Chris Dufton

Checked: May 2010

Note: does not appear to be correct plasmid

Plasmid Name: pRSETb-mycNotB4                               Stock #: 275

Strain:DH5α

Description: contains myc tag (subcloned from pT7*myc plasmid)

Created by: Mike Mazzi

Checked: May 2010

Plasmid Name: pRSetb-mycScaB4                                 Stock #: 276

Strain:DH5α

Description: contains myc tag

Created by: Mike Mazzi

Checked: May 2010

Plasmid Name: pRSetb-mycProxB4                               Stock #: 277

Strain:DH5α

Description: for protein purification 

Created by: Mike Mazzinie

Checked: May 2010

Plasmid Name: pRSetb-B4-Total tail                              Stock #: 278

Strain:DH5α

Description: for protein purification 

Created by: Mike Mazzinie

Checked: May 2010

Plasmid Name: pT7mouseVimDesmin-myc                   Stock #: 279

Strain:DH5α

Description: the head of mouse vimentin on the body of mouse desmin

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pCS2mG-XCadtail-stop                         Stock #: 280

Strain:DH5α

Description: a stopped version of the XCADTAIL plasmid

Created by: Gerry wilde

Checked: May 2010

Plasmid Name: pCS2mG-XSlugΔXba                            Stock #: 281

Strain:DH5α

Description: a version of slug with a deletion from the Xba I site to the end

Created by: Andy Ryscavage

Checked: May 2010

Plasmid Name: pCsCnxN3Δ-HPg-GFP                          Stock #: 282

Strain:DH5α

Description: I was told by the NIH boys that this contains full length CnxHPg as well. Be careful to check

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pCsCnxN4Δ-HPg-GFP                          Stock #: 283

Strain:DH5α

Description: the N4 version of the GFP-Tagged form

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pSP64T-myoD-GR                                Stock #: 284

Strain:DH5α

Description: the glucocorticoid repressor domain attached to mycD

Created by: from Hazel Sive

Checked: May 2010

Plasmid Name: pSP64T-myoD-ER                                 Stock #: 285

Strain:DH5α

Description: the estrogen repressor domain attached to myoD

Created by: from Hazel Sive

Checked: May 2010

Plasmid Name: pCsCnxN3Δ-HPg-stop                          Stock #: 286

Strain:DH5α

Description: a stopped form of N3D

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pRSETb*-XPg                                       Stock #: 287

Strain:Unknown

Description: for protein purification-Xpg cloned from CNX.Xpg.GFP

Created by: Amy

Checked: May 2010

Plasmid Name: pCsCnxN2Δ-HPg-stop                           Stock #: 288

Strain:DH5α

Description: the green form

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pCsCnxN4Δ-HPg-stop                           Stock #: 289

Strain:DH5α

Description: a stopped version of N4

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pCS2mt-ΔG-XβCAT-GR                       Stock #: 290

Description: the GR domain attached to the delta G form of Xbcat

Created by: Eric Jensen

Checked: May 2010

Plasmid Name: pMX51-cDNA XSna                             Stock #: 291

Description: the cDNA for Xenopus Snail (for insitu)

Created by: Michael Sargent

Checked: May 2010

Plasmid Name: pMX363-partail XSlug                          Stock #: 292

Strain:DH5α

Description: EcoRI fragment for Slug specific probe synthesis

Created by: Michael Sargent

Checked: May 2010

Plasmid Name: pMx384.13-pSP72XSlug                       Stock #: 293

Strain:DH5α

Description: full length cDNA for Slug

Created by: Michael Sargent

Checked: May 2010

Plasmid Name: pCS2mtΔG-Y656P-XPg                        Stock #: 294

Strain:DH5α

Description: mutated form of Xenopus plakoglobin at a likely tyrosine phosporylation site

Created by: Chris Dufton

Checked: May 2010

Plasmid Name: pCS2mt-XSlug                                       Stock #: 295

Strain:DH5α

Description: the C-terminally tagged form

Created by: andy ryscavage

Checked: May 2010

Plasmid Name: pAC-CMV-pLpA                                   Stock #: 296

Strain:DH5α

Description: grows very poorly in LB-use superbroth

Created by:

Checked: May 2010

Plasmid Name: pDNA3-S37F-BCAT-ER*                     Stock #: 297

Strain:DH5α

Description: a point mutant, activated of bCAT attached to a tamoxifen-responsive ER element

Created by: NIH boys

Checked: May 2010

Plasmid Name: pT7CnxN3Δ-HPg-GFP                          Stock #: 298

Strain:DH5α

Description: for making RNA

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: May 2010

Plasmid Name: pT7CnxN4Δ-HPg-GFP                          Stock #: 299

Strain:DH5α

Description: for making RNA

Created by: Yanni Vourgourakis- Klymkowsky lab

Checked: May 2010

Plasmid Name: pRSETb*-DsgTail                                  Stock #: 300

Strain:DH5α

Description: for protein purification

Created by: Amy Hopkins

Checked: May 2010

Plasmid Name: pCS2mtΔGXbCAT-RT comp.               Stock #: 301

Strain:DH5α

Description: this is the competitor for bCAT rtPCR (quantitative)

Created by: Chris Dufton

Checked: May 2010

Plasmid Name: pCS2mt-Siamois                    .               Stock #: 302

Strain:DH5α

Description: from the gumbiner lab

Created by: Chris Dufton

Checked: May 2010

 

Plasmid Name: pCS2-HA-XSox17β                    .          Stock #: 303

Strain:DH5α

Description: transferred to HA-XSox17b sequence from pT7 (from Aaron Zorn) to pCS2mt deleted out mt sequence

Created by: Klymowsky

Checked: May 2010

 

Plasmid Name: pCS2-HA-XSox17β (stopped)               Stock #: 304

Strain:DH5α

Description: derived from the pCS2mt-XSox17b-GFP constructed—stopped using Xba I/Klenow

Created by: Klymowsky

Checked: May 2010

Plasmid Name: pSK+XSox2                                           Stock #: 305

Strain:DH5α

Description: contains XSox2 doing region in bluescripe SK+cut with Xba I and make antisense RNA using T7 but with EcoRV and make sense RNA using T3

Created by: from the Grainger lab

Checked: May 2010

Plasmid Name: pCS2mGFP-XTcf-ΔTail                        Stock #: 306

Strain:DH5α

Description: Tail less XTcf3-Does not but with Xba I due to Dam methylation

Created by: Yanni Vourgourakis

Checked: May 2010

Plasmid Name: pACCMV- β-catenin*                            Stock #: 307

Strain:DH5α

Description: β-catenin with S37F mutation cloned into the Kpn I/Sal I sites of pACCMV

Created by: Eric Jensen

Checked: May 2010

Plasmid Name: pACCMV-β-catenin*-Δtail                    Stock #: 308

Strain:DH5α

Description: β-catenin ΔC-Terminal Tail (with S37F mutation) cloned into the Kpn I/Sal I sites of pACCMV

Created by: Eric Jensen

Checked: May 2010

Plasmid Name: pGEX-6P-3                                            Stock #: 309

Strain:DH5α

Description: GST-fusion plasmid from Pharmacia 

Created by: Pharmacia

Checked: May 2010

Plasmid Name: pCS2mt-PpnHead-stop                           Stock #: 310

Strain:DH5α

Description: plakophilin head stopped-remade in August 98-correct!!! 

Created by: Mike Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mt-XSlug-ΔXba                            Stock #: 311

Strain:DH5α

Description: myc tagged xenopus slug with the xba deletion  

Created by: Andy Ryscavage-Klymkowsky lab

Checked: May 2010

Plasmid Name: pGEX6PXSlug                                       Stock #: 312

Strain:DH5α

Description: slug in GST bacterial sythsesis vector  

Created by: Andy Ryscavage-Klymkowsky lab

Checked: May 2010

Plasmid Name: pCS2mt-ΔN166-XTcf3                          Stock #: 313

Strain:DH5α

Description: the stopped version of the DN166 XTcf3 pCS2mt plasmid

Created by: MWK

Checked: May 2010

Plasmid Name: pCDNA-HA-XTCF3                              Stock #: 314

Strain:DH5α

Description: an HA-tagged form of XTCF3 in the pCDNA plasmid

Created by: Clevers lab

Checked: May 2010

Plasmid Name: pCDNA-HA-ΔN-XTCF3                       Stock #: 315

Strain:DH5α

Description: an HA-tagged form DN-XTCF3

Created by: Clevers lab

Checked: May 2010

Plasmid Name: pGEX6p-XPg-GFP                                Stock #: 316

Strain:DH5α

Description: the xenopus plakoglobin-GFP coding sequence in the GST fusion plasmid

Created by: Amy Hopkins

Checked: May 2010

Plasmid Name: pGEX6p-XTCF3                                    Stock #: 317

Strain:DH5α

Description: the xenopus XTCF3 sequence in the GST fusion plasmid

Created by: Traci Karmazin

Checked: May 2010

Plasmid Name: pGEX6p-XPg-stop                                 Stock #: 318

Strain:DH5α

Description: the xenopus plakoglobin coding sequence in the GST fusion plasmid

Created by: Amy Hopkins/Jarmei Chen

Checked: May 2010

Plasmid Name: pMX5.3*cDNA*XSna*sp72                 Stock #: 319

Strain:DH5α

Description: full length XSnail plasmid 1.8kB EcoRI fragment T7 sense/SP6 anti sense

Created by: Michael Sargent

Checked: May 2010

Plasmid Name: pSiamois -01234-LUC                           Stock #: 320

Strain:DH5α

Description: the Siamoise promoter plasmid (driving fire fly luciferase)

Created by: Kimmelman Lab

Checked: May 2010

Plasmid Name: pSiamois (-)LUC                                    Stock #: 321

Strain:DH5α

Description: the siamois promoter with mutated TCF sites driving luciferse

Created by: Kimmelman Lab

Checked: May 2010

Plasmid Name: pTOPFLASH                                         Stock #: 322

Strain:DH5α

Description: three TCF binding sites driving fire fly luciferase  

Created by: Korinek et al

Checked: May 2010

Plasmid Name: pFOPFLASH                                          Stock #: 323

Strain:DH5α

Description: the mouse SOX10 cDNA 

Created by: obtain from Grant Barish

Checked: May 2010

Plasmid Name: pmouse Sox10                                        Stock #: 324

Strain:DH5α

Description: the mouse SOX10 cDNA 

Created by: obtain from Grant Barish

Checked: May 2010

Plasmid Name: pmouse Sox10 Dom                               Stock #: 325

Strain:DH5α

Description: the mouse SOX10 dominant mutation

Created by: obtain from Grant Barish

Checked: May 2010

Plasmid Name: pCRS.XCAD11                                      Stock #: 326

Strain:DH5α

Description: contains 1200 bp cadherin-11 sequence for antisense cut with sac I using T7 for sense, cut with Xho and use T3/

Created by: Doris Wedlich 

Checked: May 2010

Plasmid Name: pCDNA-myc-XLEF1                             Stock #: 327

Strain:DH5α

Description: the full length cDNA of Xenopus LEF-1

Created by: Miranda Molenaar 

Checked: May 2010

Plasmid Name: pPS1-HA                                                Stock #: 328

Strain:DH5α

Description: human presenilin-1 with a C-terminal HA tag

Created by: Ken Kosik lab 

Checked: May 2010

Plasmid Name: pGEX6p-XβCATENIN                          Stock #: 329

Strain:DH5α

Description: the xenopus b-catenin sequence in the GST-fusion plasmid

Created by: Jarmei Chen-Klymkowsky

Checked: May 2010

Plasmid Name: pCS2mt-Desmoglein tail                        Stock #: 330

Strain:DH5α

Description: the desmoglein tail form pT7-DgITail-Subcloned EcoRI/XBA

Created by: Chris Dufton-Klymkowsky lab

Checked: May 2010

Plasmid Name: pCS2-mt-C10Δ-Human Plakoglobin     Stock #: 331

Strain:DH5α

Description: C10D deletion of human plakoglobin, subcloned into pCS2mt

Created by: Chris Dufton-Klymkowsky lab

Checked: May 2010

Plasmid Name: pCDNA6-XSOX17b-V5-His                 Stock #: 332

Strain:DH5α

Description: from Grant Barish

Created by: Grant Barish-Varmus Lab

Checked: May 2010

Plasmid Name:pCDNA6-XSOX17β Δ 119-373-V5His Stock #: 333

Strain:DH5α

Description: deletion of tail domain

Created by: Grant Barish-Varmus Lab

Checked: May 2010

Plasmid Name:pCDNA6-XSOX17β Δ 119-373-V5His Stock #: 334

Strain:DH5α

Description: mouse LEF1 in the pCDNA6 plasmid with C-terminal V5-HIS tag

Created by: Grant Barish-Varmus Lab

Checked: May 2010

Plasmid Name:pCDNA6-human TCF4-V5-His              Stock #: 335

Strain:DH5α

Description: human TCF4 in pCDNA with a C-terminal V5-His tag

Created by: Grant Barish-Varmus Lab

Checked: May 2010

Plasmid Name:pCS2mt-Xenopus Dishvelled                 Stock #: 336

Strain:DH5α

Description: the xenopus disheveled coding sequence in the pCS2mt plasmid

Created by: Sergei Sokal

Checked: May 2010

Plasmid Name:pCS2mt-mouse Dishvelled-1                  Stock #: 337

Strain:DH5α

Description: mouse dishvelled 1 in an expression vector

Created by: from Grant Barish

Checked: May 2010

Plasmid Name:pSVK -mouse Dishvelled-2                    Stock #: 338

Strain:DH5α

Description: mouse dishevelled

Created by: from Grant Barish

Checked: May 2010

Plasmid Name:pSV-mouse Dishvelled-3                        Stock #: 339

Strain:DH5α

Description: mouse disheveled-3

Created by: from Grant Barish

Checked: May 2010

Plasmid Name:pHAT-EK-10                                          Stock #: 340

Strain:DH5α

Description: histidine-affinity tag-enterokinase cleavage site plasmid for generating tagged proteins in bacteria

Created by: CloneTech

Checked: May 2010

Plasmid Name:pSV -mouse Dishvelled-2                       Stock #: 341

Strain:DH5α

Description: mouse disheveled-2

Created by: from Grant Barish

Checked: May 2010

Plasmid Name:pCS2mt-Human plakoglobin C13Δ        Stock #: 342

Strain:DH5α

Description: mouse disheveled-2

Created by: Chris Dufton- Klymkowsky lab

Checked: May 2010

Plasmid Name:pCDNA-XSOX3-V5His                         Stock #: 343

Strain:DH5α

Description: XSOX3 with a C-Termina V5His tag

Created by: Grant Barish-Varmus lab

Checked: May 2010

Plasmid Name:pCDNA3-S28A-human plakoglobin      Stock #: 344

Strain:DH5α

Description: mutated form of human plakoglobin

Created by: Grant Barish

Checked: May 2010

Plasmid Name:pRK5-GSK3β-HA                                   Stock #: 345

Strain:DH5α

Description: I think this came from the Varmus lab

Created by: who knows?

Checked: May 2010

Plasmid Name:pCDNA3-S37A-β-catenin                       Stock #: 346

Strain:DH5α

Description: stabilized form of human b-catenin

Created by: Grant Barish

Checked: May 2010

Plasmid Name:pH4x2-LUFICERASE                            Stock #: 347

Strain:DH5α

Description: SOX response elements in from of luciferse

Created by: Aaron Zorn/Grant Barish

Checked: May 2010

Plasmid Name:pGEM-T-Rat Rho                                   Stock #: 348

Strain:DH5α

Description: has subcloned rat rhoB RT-PCR product

Created by: Eric Jensen-Klymkowsky lab

Checked: May 2010

Plasmid Name:pCD2mt-Xenopus Dishevelled               Stock #: 349

Strain:DH5α

Description: xenopus disheveled with an N-terminal myc tag

Created by: Sergai Sokoi (duplicate 7)

Checked: May 2010

Plasmid Name:pXTwin Luciferase                                 Stock #: 350

Strain:DH5α

Description: Xtwin promoter construct driving Luciferase

Created by: Ken Cho et al.

Checked: May 2010

Plasmid Name:pRC-RSV                                                Stock #: 351

Strain:DH5α

Description: Neomyocin-selectable plasmid with RSV prometer

Created by: INVITROGEN

Checked: May 2010

Plasmid Name:pRC-RSV*                                              Stock #: 352

Strain:DH5α

Description: Xba site removed

Created by: Klymkowsky

Checked: May 2010

Plasmid Name:pCD2mt-ΔDesmoplakin-GFP                Stock #: 353

Strain:DH5α

Description: dominant negative mutant form of desmoplakin (human I think)

Created by: Cathy Green

Checked: May 2010

Plasmid Name:pRcRSV-MT-Humanplakoglobin-CΔ13Stock #: 354

Strain:DH5α

Description: inserted Hind III/Not I – low level expression plasmid

Created by: Klymkowsky

Checked: May 2010

Plasmid Name:pCS2-Luciferase                                      Stock #: 355

Strain:DH5α

Description: cloned using BamHI and Xba I 

Created by: Fahlm Ghafoori-Klymkowsky lab

Checked: May 2010

Plasmid Name:pCS2mt-ΔG-X b-Catenin                        Stock #: 356

Strain:10F’

Description: same as stock 247 in E.coli strain designed for more toxic proteins grows better than 247 

Created by: Amy Hopkins-Klymkowsky lab

Checked: May 2010

Plasmid Name:pCDNA6A-V5His                                   Stock #: 357

Strain:DH5α

Description: Expression plasmid

Created by: INVITROGEN

Checked: May 2010

Plasmid Name:pCDNA6-GcR-V5-His                           Stock #: 358

Strain:DH5α

Description: Expression plasmid with subcloned glucocorticoid repressor domain

Created by: Klymkowsky

Checked: May 2010

Plasmid Name: pCDNA6-HA2-XTCF3-GcR-V5His     Stock #: 359

Strain:DH5α

Description: Expression plasmid

Created by: Klymkowsky

Checked: May 2010

Plasmid Name:pCDNA6-mycXenopus Lef-1-GcR-V5His      Stock #: 360

Strain:DH5α

Description: Xenopus LEF1 in the GcRespressor plasmid

Created by: Klymkowsky

Checked: May 2010

Plasmid Name:pCS2-XSOXD                                         Stock #: 361

Strain:DH5α

Description: supplied by Sasai

Created by: Sasai

Checked: May 2010

Plasmid Name:pCS2-GFP*                                             Stock #: 362

Strain:DH5α

Description: GFP with myc-tag removed by Cla /I EcoRI digest and blunt ligation

Created by: Eric Jensen

Checked: May 2010

Plasmid Name:pCS2-Siamois minus 3’ UTR                 Stock #: 363

Strain:DH5α

Description: pCS2Siamois cut with Xba I-SACII. Filled in with religated digest with Nco I for linearization reaction

Created by: Shoran Rose-Klymkowsky lab

Checked: May 2010

Plasmid Name:pCDNA6-mycSlug-GcR-V5-His            Stock #: 364

Strain:DH5α

Description: myc tagged with C-terminal GcRepressor-V5-His tags

Created by: Andy Ryscavage-Klymkowsky lab

Checked: May 2010

Plasmid Name:pCS2mt-COG-2                                      Stock #: 365

Strain:DH5α

Description: C.elegans COG-2 cloned into pCS2mt plasmid with EcoRI/Xba I

Created by: Chuck Parsons-Klymkowsky lab

Checked: May 2010

Plasmid Name:pCDNA6A-HaΔN32-XTCF3-GcR-V5 His     Stock #: 366

Strain:DH5α

Description: C.elegans COG-2 cloned into pCS2mt plasmid with EcoRI/Xba I

Created by: Chuck Parsons-Klymkowsky lab

Checked: May 2010

Plasmid Name:pGIEF-B5                                                Stock #: 367

Strain:DH5α

Description: .XEF1a Rnaase protection probe in pGEM1 backbone protects 88bp fragment cut with Hind III transcripe probe T7

Created by: Paul Kreig

Checked: May 2010

Plasmid Name:XSOX3-V5H                                           Stock #: 368

Strain:DH5α

Description: .Subcloned XLEF1 (Cla I/Xba I) into the pCDNA-HaHa plasmid (replacing XTCF3)

Created by: Klymkowsky

Checked: May 2010

Plasmid Name:pCDNA6HA2-XSOX17β-V5H              Stock #: 369

Strain: Unknown                

Description:

Created by: Klymkowsky

Checked: May 2010

Plasmid Name:pHAT*XβCAT                                        Stock #: 370

Strain:DH5α

Description:

Created by: Klymkowsky

Checked: June 2010

Plasmid Name:pCDNA6A-Ha-XTCF3-V5His               Stock #: 371

Strain:DH5α

Description: Cloned in Hind III-Xho from pCDNA6-HA-XTCF3GRHis

Created by: Jensen

Checked: June 2010

Plasmid Name:pCDNA6A-HA-ΔN32XTCF3-V5His     Stock #: 372

Strain:DH5α

Description: Cloned in Hind III-Xho from pCDNA6-HA-ΔN32XTCF3-GR-V5His

Created by: Eric Jensen

Checked: June 2010

Plasmid Name:pCS2mt-Snail                                          Stock #: 373

Strain: Unknown                

Description: snail coding region cloned and Eco/RI XbaI (not correct plasmid)

Created by: K.WEST

Checked: June 2010

Plasmid Name:pCS2mt-Slug                                          Stock #: 374

Strain: Unknown                

Description: snail coding region cloned and Eco/RI XbaI (not correct plasmid)

Created by: Alli and Andy

Checked: June 2010

Plasmid Name:pCS2mt-XTCF3-V5his                           Stock #: 375

Strain:DH5α

Description: 5’ ClaI site is Dom sensitive XTCF3 inserted Cla Xho

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-ΔN32XTCF3-V5His                   Stock #: 376

Strain:DH5α

Description: ΔN32XTCF3 inserted Cla Xho ClaI site dom sensitive

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOX3V5His                              Stock #: 377

Strain:DH5α

Description: XSOX3=> Cla Xho

ClaI site d>m sensitivity.

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOX5V5His                              Stock #: 378

Strain:DH5α

Description: XSOX5 from Grammer=> Cla Xho ClaI not dom sensitive

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOXDV5His                             Stock #: 379

Strain:DH5α

Description: XSOXD from Grammer=> Cla Xho ClaI not dom sensitive

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOX11-V5His                           Stock #: 380

Strain:DH5α

Description: XSOX11 inserted Cla Xho

ClaI is not dom sensitive 

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOX3 ΔN-V5His                      Stock #: 381

Strain:DH5α

Description: deletion of amino acids 1-155 ClaI is Dom insensitive

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOX3 ΔC-V5His                       Stock #: 382

Strain:DH5α

Description: deletion of amino acids 1-156-310 ClaI is Dom sensitive

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pNPG152 (Nrp Probe Template)           Stock #: 383

Strain: Unknown                

Description: Cut Xba for sensitive probe Pon-neural Marker

Created by:

Checked: June 2010

Plasmid Name: p24-10 Neural-Specificβtubutin            Stock #: 384

Strain: Unknown                

Description: Cut Xba for sensitive probe transcribed T3 pan-neural marker

Created by: ?

Checked: June 2010

Plasmid Name: pSK*Hairy 2a                                         Stock #: 385

Strain: Unknown                

Description: Cut BamHI transcribed F7 for antisense probe

Created by: Dave Turner

Checked: June 2010

Plasmid Name: pGem-XSOX3                                        Stock #: 386

Strain: Unknown                

Description: Probe template Cut Sol I, transcribed SP6 for antisense

Created by: Yanni

Checked: June 2010

Plasmid Name: pBluescript-SK-XAG-1                         Stock #: 387

Strain: Unknown                

Description: Probe template Cement Gland marker

Cut Not I transcribed antisense probe T7

Created by: Hazel Sive

Checked: June 2010

Plasmid Name: pCS105XSOX5.2                                   Stock #: 388

Strain: Unknown                

Description: Probe template 3’UTR removed from original pCS105-XSOX5

Cut Sol I, Transcribed antisense probe T3

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS105-XSOX11.2                                Stock #: 389

Strain: Unknown                

Description: Probe template 3’UTR removed from original pCS105-XSOX11

Cut Sol I, Transcribed antisense probe T3

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pBluescript KS*-en2                              Stock #: 390

Strain: Unknown                

Description: en 2 probe template, Mid/Hind brain marker

Cut Xba, Transcribed T3 for antisense probes

Created by: ?

Checked: June 2010

Plasmid Name: pGem4-XKrox 20                                  Stock #: 391

Strain: Unknown                

Description: en 2 probe template, marker for rhombomers 3,5 in hind brain and neural crest adjacent to Rhomb 5. Cut EcoRI, transcribed probe T7

Cut Xba, Transcribed T3 for antisense probes

Created by: David Wilkinson, Not I institute for medical research london

Checked: June 2010

Plasmid Name: DH5 comp cells                                     Stock #: 392

Strain:DH5α

Description:

Created by: ?

Checked: June 2010

Plasmid Name: No entry for this stock                            Stock #: 393

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pGemblue-XWnt-1                                 Stock #: 394

Strain:DH5α

Description: for in situ probe but Xbe, transcribe

Created by: From O.Destree

Checked: June 2010

Plasmid Name: pCS2-XSOX9 Full length                      Stock #: 395

Strain:DH5α

Description: Cut with Not I

Created by: Shana Fawcett

Checked: June 2010

Plasmid Name: p-gen-T-easy-X-SOX9                           Stock #: 396

Strain:DH5α

Description: Cut with Sal I

Created by: Shana Fawcett

Checked: June 2010

Plasmid Name: Pax3 Probe Template                             Stock #: 397

Strain: Unknown                

Description: for in situ probe linearize Blg II and transcribe SP6

Created by: from anne Band/ Chris Kintner

Checked: June 2010

Plasmid Name: pCS2-XSOX3m7-V5His                        Stock #: 398

Strain: Unknown                

Description: HMG-Box point mutant generated by Chi Zhang

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-XSOX3m8-V5His                        Stock #: 399

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-XSOX3m52-V5His                      Stock #: 400

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-XSOX3m68-V5His                      Stock #: 401

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pSlug-mtGFP                                         Stock #: 402

Strain: Unknown                

Description: XSlug Beta Promoter cloned upstream of mtGFP, replacing the CMV promoter of pCS2*mtGFP

Created by: Eric Jensen

Checked: June 2010

Plasmid Name: pCS2-XSOX2                                         Stock #: 403

Strain: Unknown                

Description: original cDNA cloned for Sasai includes endogenous SOX2 UTR’s inserted Eco/Xho

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-MT-XSOX3ΔC-Vp16                  Stock #: 404

Strain:DH5α

Description:

Created by: ?

Checked: June 2010

Plasmid Name: No information                                       Stock #: 405

Description:

Created by: ?

Checked: June 2010

Plasmid Name: No information                                       Stock #: 406

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pEuR-XSOX3ΔC                                   Stock #: 407

Strain:DH5α

Description: insert was cloned into EcoRI

Created by: ?

Checked: June 2010

Plasmid Name: pBEND2-Xur5WT                                 Stock #: 408

Strain:DH5α

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pBEND2-Xur5MUT                              Stock #: 409

Strain:DH5α

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-NeuroD-Vp16                              Stock #: 410

Strain: Unknown                

Description:

Created by: Made by D Macora (Lee Lab)

Checked: June 2010

Plasmid Name: No information                                       Stock #: 411 Strain: Unknown                        

Description:

Created by: ?

Checked: June 2010

Plasmid Name: No information                                       Stock #: 412

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-VP16-GR                                     Stock #: 413

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-ER-GR                                         Stock #: 414

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name:XKaiso/pCS2+mt                                    Stock #: 415

Strain:DH5α

Description:

Created by: from Pierre McCrea

Checked: June 2010

Plasmid Name:XKaiso/pCS2+3HA                                Stock #: 416

Strain:DH5α

Description:

Created by: from Pierre McCrea

Checked: June 2010

Plasmid Name:XKaiso/pCS2+C-3HA                            Stock #: 417

Strain:DH5α

Description:

Created by: from Pierre McCrea

Checked: June 2010

Plasmid Name:Xath/pBlueScript SK-                             Stock #: 418

Strain:DH5α

Description:

Created by: from Brain Parr

Checked: June 2010

Plasmid Name:XDelta/72-X-Delta-1H/R                        Stock #: 419

Strain:DH5α

Description:

Created by: from Brain Parr

Checked: June 2010

Plasmid Name:XSOX10/pCS2                                        Stock #: 420

Strain: Unknown                

Description: Sense RNA-linearize with Not I transcribe with SP6 and Antisense RNA- linearize with Cla I transcribe with T7

Created by: Yiochiro aobi

Checked: June 2010

Plasmid Name:NF.Proto-cadherin                                   Stock #: 421

Strain:DH5α

Description: NFPCDelta SalI/Bs for antisense in situ: linaerize Not I, transcribe T3

Created by: R Bradley

Checked: June 2010

Plasmid Name:pCS2+FRL-1                                          Stock #: 422

Strain:DH5α

Description: Sense probe: linearize Not I, transcribe sp6, Antisense probe: linearize Cla, transcribe T7

Created by: Yabe et al

Checked: June 2010

Plasmid Name:Xerl                                                          Stock #: 423

Strain:DH5α

Description: for RNA: Not I, sp6 for antisense probe

Created by: T.Kinoshita

Checked: June 2010

Plasmid Name: pCS2-XSOX7-GFP                                Stock #: 424

Strain:DH5α

Description:

Created by: Tamara Basta

Checked: June 2010

Plasmid Name: Xenopus VEGF cDNA pBS SK+          Stock #: 425

Strain:DH5α

Description: for antisense: linearize with BamHI/transcribe T7 polymerase

Created by: Kathryn Tonissen

Checked: June 2010

Plasmid Name: ESG-pGEX-4T3                                     Stock #: 426

Strain:DH5α

Description:

Created by: Walter Knochel

Checked: June 2010

Plasmid Name: ESG-pCS2-FLAG                                  Stock #: 427

Strain:DH5α

Description:

Created by: Walter Knochel

Checked: June 2010

Plasmid Name: pSp Aol                                                  Stock #: 428

Strain:DH5α

Description: template for splicing but with HincII=> RNA synthesis from T7 promoter 

Created by: JA

Checked: June 2010

Plasmid Name: Xbcl-2 FLAG                                         Stock #: 429

Strain:DH5α

Description: mRNA; linearize BamHI; polymerize T7

Created by: Mallu lab

Checked: June 2010

Plasmid Name: OTX2/pBS                                             Stock #: 430

Strain: Unknown                

Description: sense cut with HindII, T3 and antisense cut with NotI, T7

Created by: University of Pen. Klein lab

Checked: June 2010

Plasmid Name: XMyoDb for insitu probe exclusively    Stock #: 431

Strain:DH5α

Description: pbsks+XMyoDb but with Hindui, transcribe T7

Created by: Ralph Rupp

Checked: June 2010

Plasmid Name: pCS2-n-B-gal                                         Stock #: 432

Strain:DH5α

Description: cut with NotI, transcripted by SP6 

Created by: University of Pen. Klein lab

Checked: June 2010

Plasmid Name: pRR104-XMycD6                                  Stock #: 433

Strain: Unknown                

Description: for sense RNA, linearizing Asp 718; transcribed SP6 or NotI 

Created by: Ralph Rupp

Checked: June 2010

Plasmid Name: pCS2-myc-Xsnail 2-GFP                       Stock #: 434

Strain: Unknown                

Description: cloned by Chi, (site EcoRI/XbaI 

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-myc-Xsnail 2-GFP                       Stock #: 435

Strain: Unknown                

Description: cloned by Chi, (site EcoRI/XbaI 

Created by: ?

Checked: June 2010

Plasmid Name: Human bcl2                                            Stock #: 436

Strain:DH5α

Description: for RNA: linearize StuI; transcribe T7 

Created by: Gantien lab

Checked: June 2010

Plasmid Name: pCS3-Mixer                                            Stock #: 437

Strain: Unknown                

Description: sense RNA cut with Ascl transcribe with Sp6 

Created by: Gantien lab

Checked: June 2010

Plasmid Name: pCS3-GnR-Mixer                                   Stock #: 438

Strain: Unknown                

Description:

Created by: Melton lab

Checked: June 2010

Plasmid Name: blue-ScriptII-XSox18cDNA                  Stock #: 439

Strain: Unknown                

Description: in EcoRI/Xho1 situ

Created by: Yoshiki Hiraoka

Checked: June 2010

Plasmid Name: XFGF8                                                   Stock #: 440

Strain: Unknown                

Description: Lineaization Xba1 (initu probe) T3 for antisense RNA

Created by: ?

Checked: June 2010

Plasmid Name: VegT-Eng SacII sp6                               Stock #: 441

Strain: Unknown                

Description: Lineaization Xba1 (initu probe) T3 for antisense RNA

Created by: ?

Checked: June 2010

Plasmid Name: pCs2-mt-XPygopus                                Stock #: 442

Strain: Unknown                

Description: in NcoI/EcoRI Acc.AF521655

Created by: Belenkaya et al. (2002) Dev. 129(17) 40894101

Checked: June 2010

Plasmid Name:p(SK-)-Squint (Fish)                               Stock #: 443

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name:p(SK-)-Cyclos (Fish)                              Stock #: 444

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name:pCS2-XSOX7ALT-GFP(in ClaI/XbaI)  Stock #:445

Strain:DH5α

Description: alternative from of XSOX7=>for SOX7MO Rescue

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-XSOX18ALT-GFP(in ClaI/XbaI)Stock #:446

Strain:DH5α

Description: alternative from of XSOX7=>for SOX7MO Rescue

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:GBx2a                                                    Stock #: 447

Strain: Unknown                

Description: anti-sense BamHI T7

Created by: Shalom Graciela’s lab

Checked: June 2010

Plasmid Name:pCS2-XSOX1-V5H                                Stock #: 448

Strain: Unknown                

Description: for sense RNA SACII linearization, Sp6 transcription

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:pCS2-XSox7ΔC-GFP                             Stock #: 449
Strain:DH5α

Description: in ClaI/EcoRI contains HMG box +20ava

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-Sox1-GFP                                      Stock #: 450

Strain:DH5α

Description: in ClaI/EcoRI

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2+MT (original plasmid)                  Stock #: 451

Strain: Unknown                

Description:

Created by: Harland lab

Checked: June 2010

Plasmid Name:BCLg in pBSIISK backbone                   Stock #: 452

Strain: Unknown                 

Description: GenBanc Acc.No.XM_563

Created by: Thomas Kramps

Checked: June 2010

Plasmid Name:pTK1-mtBclg                                          Stock #: 453 Strain:DH5α

Description: 6myc emitope from pCS2+mt vector was cloned in front of pIK1Balg. For Cap RNA linaerize w SacI and aux.w.Sp6

Created by: Tamara Basta

Checked: June 2010

Plasmid Name: pCS2-GR-XSox7-GFP                           Stock #: 454

Strain:DH5α

Description: GR insert was cloned in front of pCS2-AltXSox7-GFP into BamHI ClaI sites. In this construct Cla is dam sensitive

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-GR-XSox7ΔC-VP16                     Stock #: 455

Strain: Unknown                

Description: XSox7ΔC(alt) insert cloned into BamHI/EcoRI site)

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-GR-XSox18ΔC-VP16                   Stock #: 456

Strain: Unknown                

Description: XSox18ΔC insert cloned into BamHI/EcoRI site)

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-GR-XSox3ΔC-VP16                     Stock #: 457

Strain: Unknown                

Description: XSox3ΔC insert was cloned BamHI EcoRI sites. This clone is frame with Myc with VP16 

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-GR-Slug-GFP                                Stock #: 458

Strain: Unknown                

Description: (slug was inserted in pCS2-GR-GFP with ClaI :: XbaI site ClaI is dam sensitive ) to make injected RNA linearized by SACII

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:pCS2-GR-XSox3-GFP                            Stock #: 459

Strain: Unknown                

Description: XSox3C insert cloned into pCS2-GR-GFP with ClaI :: XbaI site to make injected RNA linearized by SacII or NotI transcribe with Sp6

Created by:

Checked: June 2010

Plasmid Name:pCS2-UTR-XSox18-V5H                       Stock #: 460

Strain: Unknown                

Description: (5’ UTR-XSOX18 was inserted into pCS2-V5H was Cla2::XhoI to make injected RNA linearized with NotI transcribe with Sp6

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:C-MYC (295D8)                                     Stock #: 461

Strain: Unknown                

Description: cut and transcribe with for probe antisense EcrI/T7

Created by: La Bonne lab

Checked: June 2010

Plasmid Name:MyoCardin                                              Stock #: 462

Strain: Unknown                

Description: cut with BamHI Transcribed with Sp6 for insitu probe

Created by: Pan A.Kreig

Checked: June 2010

Plasmid Name:BKBlue (reported) in pGL3                    Stock #: 463

Strain: Unknown                

Description:

Created by: ?

Checked: June 2010

Plasmid Name:1KBSA-mutant                                        Stock #: 464

Strain: Unknown               

Description: 1KBd 532A/536A (pCDNA) from 1CS

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-ΔN35XSOX3                                Stock #: 465

Strain: Unknown                

Description: (ClaI/EcoRI insert (PCR generated) of ΔN35XSOX3 cloned into pCS2-GFP vector. Insert contains stop codon (GM2163 cells)

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-ΔN35XSOX3.GFP                        Stock #: 466

Strain: Unknown                

Description: (ClaI/EcoRI insert (PCR generated) of ΔN35XSOX3 cloned into pCS2-GFP vector. Insert have no stop codon (into GM2163 cells) NOT GREEN

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pSP64T:XrelA                                         Stock #: 467

Strain: Unknown               

Description: cut by EcoRI, transcribed with Sp6

Created by: ?

Checked: June 2010

Plasmid Name:pSP64T:XrelAΔSP                                  Stock #: 468

Strain: Unknown               

Description: cut by EcoRI, transcribe with Sp6

Created by: Hugh Woodland

Checked: June 2010

Plasmid Name:pCS2-XrelA                                            Stock #: 469

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name:pCS2-GR-XR11-GFP(in GM2163 cells)Stock #: 470

Strain: GM2163                

Description: contains N-terminal GRE domain Xenopus XR11 (GuyBack #X82461) and C-Termanial GFP NheI/XbaI between XR11 and GFP

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-ΔN35XSOX3V5(in GM2163cells) Stock#:471

Strain: GM2163               

Description:

Created by: Tamara Basta

Checked: June 2010

Plasmid Name:pCS2-GR-XRelA                                    Stock #: 472

Strain: Unknown              

Description: GR  was inserted into ClaI site in front of XRalA)

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:pU52-eGFP                                             Stock #: 473

Strain: Unknown              

Description:

Created by: Jack Parent university of Michigan

Checked: June 2010

Plasmid Name:pCS2-X1KB-V5H                                   Stock #: 474

Strain: Unknown              

Description: (X2KB who inserted into BamH : Xho2 site) Linearized by NotI transcribed with Sp6 for RNA

Created by: Jack Parent university of Michigan

Checked: June 2010

Plasmid Name:pCS2-WnD (Fly)-V5H                            Stock #: 475

Strain: Unknown              

Description: (X2KB who inserted into BamH : Xho2 site) Linearized by NotI transcribed with Sp6 for RNA

Created by: Jack Parent university of Michigan

Checked: June 2010

Plasmid Name:5Cl                                                           Stock #: 476

Strain: Unknown              

Description: T7/Xho2 gives antisense for insitu

Created by: Smith lab

Checked: date…

Plasmid Name:pUS2-GR-eGFP                                      Stock #: 477

Strain: Unknown              

Description: GR was inserted into AatII site constructed by 

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:pGBk-XSOX7                                         Stock #: 478

Strain: Unknown              

Description: T7/SPEI give anti-sense probe

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:pUS2-GR-eGFP                                      Stock #: 479

Description: GR was inserted into AatII site of pUS2-eGFP. Note: pDNA injection in Xenopus can see green.

Created by: Chi Zhang

Checked: June 2010

Plasmid Name:pG3 cytoskeleton Cepidermal                 Stock #: 480

Strain: Unknown             

Description: anti-sense BamHI/Sp6

Created by: Harland

Checked: June 2010

Plasmid Name:pME-XrelB                                              Stock #: 481

Strain: Unknown              

Description: anti-sense BamHI/Sp6

Created by: Kazuo Marayama University of Tokyo

Checked: June 2010

Plasmid Name:pDSRed monomer                                   Stock #: 482

Strain: Unknown              

Description:

Created by: Clonetech

Checked: June 2010

Plasmid Name:pDSRed2                                                  Stock #: 483

Strain: Unknown              

Description:

Created by: Clonetech

Checked: June 2010

Plasmid Name:pXR11.DsRed monomer                         Stock #: 484

Strain: Unknown              

Description:

Created by: Clonetech

Checked: June 2010

Plasmid Name:pCS107-XSOX2                                      Stock #: 485

Strain: Unknown              

Description: sense Sp6 Ascl anti-sense T7 HindII

Created by: Clonetech

Checked: June 2010

Plasmid Name:pCS107-XSOX3                                      Stock #: 486

Strain: Unknown              

Description: sense Sp6 Ascl anti-sense T7 HindII

Created by: Clonetech

Checked: June 2010

Plasmid Name:pUS2-eGTP/Sal-                                     Stock #: 487

Strain: Unknown              

Description:

Created by: Clonetech

Checked: June 2010

Plasmid Name:pERTM-XR11-DsRedm                         Stock #: 488

Strain: Unknown              

Description:

Created by: Clonetech

Checked: June 2010

Plasmid Name:pUS2 eGFP/SAL- Not- (Nsi/Not-)         Stock #: 489

Strain: Unknown              

Description:

Created by: Clonetech

Checked: June 2010

Plasmid Name:pUS eGFP/SAL- Not- (Nsi/Not-)           Stock #: 490

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name:pUS2-GR-SOX3-GFP                             Stock #: 491

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name:pUS2-GR-SOX7-GFP                             Stock #: 492

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name: No information                                       Stock #: 493

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCDNA3                                                 Stock #: 494

Strain: Unknown              

Description: in Dh5a no insert

Created by: ?

Checked: June 2010

Plasmid Name: SOX1ΔUTR (CS108)                             Stock #: 495

Strain: Unknown              

Description:

Created by: Tim grammer

Checked: June 2010

Plasmid Name: Squint in SK                                           Stock #: 496

Strain: Unknown              

Description: Sense Asp 718/13, antisense Zcor1/T7

Created by: Chris Wright

Checked: June 2010

Plasmid Name: pCDNA-GR                                            Stock #: 497

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCDNA-GFP                                          Stock #: 498

Strain: Unknown             

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCDNA-GRGFP                                    Stock #: 499

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCDNA-GR-SOX3-GFP                        Stock #: 500

Strain: Unknown             

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCDNA-GR-SOX7-GFP                        Stock #: 501

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name: Xpat                                                        Stock #: 502

Strain: Unknown              

Description: AS cut Eco R1, transcribe with T7, Sense: cut XhoI, transcribe T3

Created by: Woodland lab

Checked: June 2010

Plasmid Name: pCS2-Oct 25 SP6/Not!                           Stock #: 503

Strain: Unknown              

Description:

Created by: Dr. Ying Lao

Checked: June 2010

Plasmid Name: pCS2-Oct 60 SP6/Not!                           Stock #: 504

Strain: Unknown              

Description:

Created by: Dr. Ying Lao

Checked: June 2010

Plasmid Name: pCS2-Oct 91 SP6/Not!                           Stock #: 505

Strain: Unknown              

Description:

Created by: Dr. Ying Lao

Checked: June 2010

Plasmid Name: pCS2-SOX3-GFP                                   stock #: 506

Strain: Unknown              

Description: 5’Cla1/3’Xba1 site. VERY GREEN

Created by: Chi Zhang

Checked: June 2010

Plasmid Name: pCS2- ΔN35XSOX3-GFP                      Stock #: 507

Strain: Unknown              

Description: 5’Cla1/3’Xba1 site. VERY GREEN

Created by: Chi Zhang

Checked: June 2010

Plasmid Name: pIRES-BASE PLASMID                       Stock #: 508

Strain: Unknown              

Description:

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-Xolloid                                         Stock #: 509

Strain: Unknown              

Description: Not1/Sp6 some RNA

Created by: ?

Checked: June 2010

Plasmid Name: pCS2-Cerberus                                       Stock #: 510

Strain: Unknown              

Description: EcoR1/T7 antisense RNA

Created by: ?

Checked: June 2010

Plasmid Name: pIRES2::GFP KanR                               Stock #: 511

Strain: Unknown             

Description:

Created by: Arthur Guiter Houtmen

Checked: June 2010

Plasmid Name: pIRES2.GFPSOX7::GFP KanR             Stock #: 512

Strain: Unknown              

Description:

Created by: MK

Checked: June 2010

Plasmid Name: pIRES2::GFP AmpR                              Stock #: 513

Strain: Unknown             

Description:

Created by: MK

Checked: June 2010