Xenopus embryonic nuclear isolation, & RNA run-on method
Table of Methods
embryo stained in wholemount for exongenous RelA

nuclear isolation form embryos ala Farzaneh & Pearson (REF)
  1. in preparation

  2. 2.5M sucrose solution
    2.5 M sucose
    2 mM MgCl2
    5 mM-Tris-HCl pH 8-0 at 2°C
    2 mM 2-mercaptoethanol
    0.5 mM spermine
    0.4% (w/v) Triton-N101 (Sigma Chemical),

    Embryo wash :
    1ml 140 mM NaCl
    10 mM Tris pH 7.4

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RNA run on analysis:

in preparation

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Table of Methods  
revised 24-Oct-2008